Identifying molecular targets for modulating carotenoid accumulation in rice grains

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
{"title":"Identifying molecular targets for modulating carotenoid accumulation in rice grains","authors":"","doi":"10.1016/j.bbrep.2024.101815","DOIUrl":null,"url":null,"abstract":"<div><p>Carotenoids are potential antioxidants offering extensive human health benefits including protection against chronic diseases. Augmenting the supply of health-benefiting compounds/metabolites through dietary supplements is the most sustainable way for a healthy life. Our study compares the traditional rice cultivar Kavuni and the white rice variety ASD 16. RNA-Seq analysis was carried out in the maturing panicles of Kavuni, which are enriched with antioxidants such as the therapeutic carotenoid lutein, polyphenols, and anthocyanins, along with “ASD 16”, a popularly eaten white rice variety, to elucidate the molecular networks regulating accumulation of health benefiting compounds. Systematic analysis of transcriptome data identified preferential up-regulation of carotenoid precursors (<em>OsDXS</em>, <em>OsGGPS</em>) and key carotenoid biosynthetic genes (<em>OsPSY1</em>, <em>OsZ-ISO</em>) in the maturing grains of Kavuni. Our study also identified enhanced expression of <em>OsLYC-E</em>, <em>OsCYP97A,</em> and <em>OsCYP97C</em> transcripts involved in the alpha-carotenoid biosynthetic pathway and thereby leading to elevated lutein content in the grains of Kavuni. Kavuni grains showed preferential down-regulation of negative regulators of carotenoid metabolism viz., AP2 and HY5 and preferential up-regulation of positive modulators of carotenoid metabolism viz., <em>Orange</em>, <em>OsDjB7,</em> and <em>OsSET29</em>, thus creating a favorable molecular framework for carotenoid accumulation. Our study has unearthed valuable gene control points for precise manipulation of carotenoid profiles through CRISPR-based gene editing in rice grains. Perturbation of carotenoid biosynthesis holds unprecedented potential for the rapid development of the next generation of ‘Golden rice’.</p></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405580824001791/pdfft?md5=9c44fab5940a2f799c998f97f4af1b36&pid=1-s2.0-S2405580824001791-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry and Biophysics Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405580824001791","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Carotenoids are potential antioxidants offering extensive human health benefits including protection against chronic diseases. Augmenting the supply of health-benefiting compounds/metabolites through dietary supplements is the most sustainable way for a healthy life. Our study compares the traditional rice cultivar Kavuni and the white rice variety ASD 16. RNA-Seq analysis was carried out in the maturing panicles of Kavuni, which are enriched with antioxidants such as the therapeutic carotenoid lutein, polyphenols, and anthocyanins, along with “ASD 16”, a popularly eaten white rice variety, to elucidate the molecular networks regulating accumulation of health benefiting compounds. Systematic analysis of transcriptome data identified preferential up-regulation of carotenoid precursors (OsDXS, OsGGPS) and key carotenoid biosynthetic genes (OsPSY1, OsZ-ISO) in the maturing grains of Kavuni. Our study also identified enhanced expression of OsLYC-E, OsCYP97A, and OsCYP97C transcripts involved in the alpha-carotenoid biosynthetic pathway and thereby leading to elevated lutein content in the grains of Kavuni. Kavuni grains showed preferential down-regulation of negative regulators of carotenoid metabolism viz., AP2 and HY5 and preferential up-regulation of positive modulators of carotenoid metabolism viz., Orange, OsDjB7, and OsSET29, thus creating a favorable molecular framework for carotenoid accumulation. Our study has unearthed valuable gene control points for precise manipulation of carotenoid profiles through CRISPR-based gene editing in rice grains. Perturbation of carotenoid biosynthesis holds unprecedented potential for the rapid development of the next generation of ‘Golden rice’.

确定调节稻谷类胡萝卜素积累的分子靶标
类胡萝卜素是一种潜在的抗氧化剂,对人体健康具有广泛的益处,包括预防慢性疾病。通过膳食补充剂增加有益健康的化合物/代谢物的供应量,是实现健康生活的最可持续的方法。我们的研究比较了传统水稻品种 Kavuni 和白米品种 ASD 16。我们对富含抗氧化剂(如治疗性类胡萝卜素叶黄素、多酚和花青素)的 Kavuni 和广受欢迎的白米品种 "ASD 16 "的成熟圆锥花序进行了 RNA-Seq 分析,以阐明调控有益健康化合物积累的分子网络。对转录组数据的系统分析发现,类胡萝卜素前体(OsDXS、OsGGPS)和关键类胡萝卜素生物合成基因(OsPSY1、OsZ-ISO)在卡武尼成熟谷粒中优先上调。我们的研究还发现,参与α-类胡萝卜素生物合成途径的 OsLYC-E、OsCYP97A 和 OsCYP97C 转录本表达增强,从而导致卡武尼谷粒中叶黄素含量升高。卡武尼谷粒中类胡萝卜素代谢负调控因子(即 AP2 和 HY5)优先下调,类胡萝卜素代谢正调控因子(即 Orange、OsDjB7 和 OsSET29)优先上调,从而为类胡萝卜素积累创造了有利的分子框架。我们的研究发现了一些有价值的基因控制点,可通过基于CRISPR的基因编辑对水稻籽粒中的类胡萝卜素含量进行精确控制。对类胡萝卜素生物合成的干扰为下一代 "黄金大米 "的快速发展带来了前所未有的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信