Immune Rejection of Cartilage in a Swine Vascularized Composite Allotransplantation Model

IF 0.8 4区医学 Q4 IMMUNOLOGY

Transplantation proceedings Pub Date : 2024-09-05 DOI:10.1016/j.transproceed.2024.08.042

Lei Zhang , Isabel Arenas Hoyos , Anja Helmer , Yara Banz , Beat Haenni , Ioana Lese , Mihai Constantinescu , Robert Rieben , Radu Olariu

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引用次数: 0

Abstract

Background

Cartilage is a crucial tissue in vascularized composite allotransplantation (VCA) and plays a pivotal role in restoring motor function, especially in joint allotransplantation. Nevertheless, our understanding of immune rejection in cartilage remains limited and contentious. This study seeks to investigate the immune rejection of cartilage in a large animal model of VCA.

Methods

Cartilage, including articular cartilage and meniscus, as well as skin, muscle and lymph node, was retrieved from a swine heterotopic VCA graft when the skin of the graft suffered from grade III-IV rejection. Histologic examination, transmission electron microscopy and immunofluorescent staining were used to investigate immune rejection.

Results

Histologic examination revealed the infiltration of inflammatory cells and tissue destruction in cartilage. Transmission electron microscopy confirmed tissue damage and necrosis in cartilage. However, cartilage exhibited milder tissue damage when compared to rejected skin and muscle. Immunofluorescent staining revealed the activation of both the innate and adaptive immune systems, accompanied by an up-regulation of cell death biomarkers, including apoptosis and pyroptosis, in the rejected cartilage.

Conclusion

Our study demonstrates that cartilage is not immunologically privileged and undergoes immune rejection concurrently with skin and muscle in the VCA graft, though with less severe inflammation and rejection.

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猪血管化复合体同种异体移植模型中软骨的免疫排斥反应

背景：软骨是血管化复合体同种异体移植（VCA）中的重要组织，在恢复运动功能方面发挥着关键作用，尤其是在关节同种异体移植中。然而，我们对软骨免疫排斥反应的了解仍然有限且存在争议。本研究旨在研究VCA大型动物模型中软骨的免疫排斥反应：方法：当猪异位 VCA 移植的皮肤出现 III-IV 级排斥反应时，从猪异位 VCA 移植中取出软骨，包括关节软骨和半月板，以及皮肤、肌肉和淋巴结。采用组织学检查、透射电子显微镜和免疫荧光染色来研究免疫排斥反应：组织学检查显示炎症细胞浸润和软骨组织破坏。透射电子显微镜证实了软骨的组织损伤和坏死。不过，与排斥反应的皮肤和肌肉相比，软骨的组织损伤较轻。免疫荧光染色显示，先天性免疫系统和适应性免疫系统均被激活，同时排斥软骨中的细胞死亡生物标志物（包括细胞凋亡和热凋亡）上调：我们的研究表明，软骨并不具有免疫特权，在VCA移植中与皮肤和肌肉同时发生免疫排斥反应，但炎症和排斥反应并不严重。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Transplantation proceedings 医学-免疫学

CiteScore

1.70

自引率

0.00%

发文量

502

审稿时长

60 days

期刊介绍： Transplantation Proceedings publishes several different categories of manuscripts, all of which undergo extensive peer review by recognized authorities in the field prior to their acceptance for publication. The first type of manuscripts consists of sets of papers providing an in-depth expression of the current state of the art in various rapidly developing components of world transplantation biology and medicine. These manuscripts emanate from congresses of the affiliated transplantation societies, from Symposia sponsored by the Societies, as well as special Conferences and Workshops covering related topics. Transplantation Proceedings also publishes several special sections including publication of Clinical Transplantation Proceedings, being rapid original contributions of preclinical and clinical experiences. These manuscripts undergo review by members of the Editorial Board. Original basic or clinical science articles, clinical trials and case studies can be submitted to the journal?s open access companion title Transplantation Reports.