Multiple PDT sessions with chlorin-e6 and LL-37 loaded-nanoemulsion provide limited benefits to periodontitis in rats

IF 3.1 3区医学 Q2 ONCOLOGY

Photodiagnosis and Photodynamic Therapy Pub Date : 2024-09-04 DOI:10.1016/j.pdpdt.2024.104329

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引用次数: 0

Abstract

Background

The combination of photodynamic therapy (PDT) and LL-37 has never been tested in an animal study and our research team background suggests this strategy might be a promising alternative to intensify periodontitis resolution. This study aimed to assess the effects of multiple sessions of PDT with chlorin-e6 conjugated to the antimicrobial peptide LL-37 loaded nanoemulsion, as adjunctive therapy in experimental periodontitis in rats.

Methods

Experimental periodontitis was induced in 81 rats. After disease establishment, animals were assigned to three groups: SRP (scaling and root planning); SRP + 1PDT, SRP followed by a single PDT session; SRP + 4PDT (n = 27), SRP followed by four PDT sessions at 0, 24, 48 and 72 h after SRP. Animals were subjected to euthanasia at 7, 14 and 28 days, and samples were submitted to osteoclast quantification, immunological and microtomography analysis.

Results

All treatments resulted in significant periodontal improvements and there was no significant difference between the groups in both local inflammatory response and healing process. Minimal adjunctive effects could be found for the combined therapy in terms of cytokine levels (IL-1β and IL-10), with no statistical significance. However, the number of TRAP-positive osteoclasts per mm of alveolar bone linear surface for the group treated with PDT sessions was significantly lower than those treated with SRP only.

Conclusions

Multiple PDT sessions with chlorin-e6 and LL-37 nanoemulsion as an adjunct to scaling and root planning reduced the presence of osteoclast in the local site but did not contribute towards bone regeneration and IL-1β and IL-10 levels.

查看原文本刊更多论文

使用氯素-e6 和 LL-37 负载纳米乳液进行多次光导照相疗法对大鼠牙周炎的治疗效果有限。

背景：光动力疗法（PDT）与 LL-37 的结合从未在动物实验中进行过测试，而我们研究团队的背景表明，这种策略可能是加强牙周炎缓解的一种有前途的替代方法。本研究旨在评估氯素-e6 与抗菌肽 LL-37 结合的纳米乳液作为实验性牙周炎的辅助疗法，对大鼠进行多次光导治疗的效果：方法：在 81 只大鼠中诱导实验性牙周炎。方法：对 81 只大鼠进行实验性牙周炎诱导：SRP（洗牙和牙根规划）；SRP + 1PDT，SRP 后进行一次 PDT 治疗；SRP + 4PDT（n=27），SRP 后进行四次 PDT 治疗，时间分别为 SRP 后的 0、24、48 和 72 小时。动物分别在 7、14 和 28 天后安乐死，并对样本进行破骨细胞定量、免疫学和显微层析成像分析：结果：所有治疗方法均能明显改善牙周状况，且各组在局部炎症反应和愈合过程中无明显差异。就细胞因子水平（IL-1β 和 IL-10）而言，联合疗法的辅助效果极小，无统计学意义。然而，接受过 PDT 治疗组每毫米牙槽骨线表面的 TRAP 阳性破骨细胞数量明显低于仅接受 SRP 治疗组：结论：使用氯素-e6 和 LL-37 纳米乳液进行多次光导治疗，作为洗牙和根面规划的辅助治疗，可减少局部部位破骨细胞的存在，但无助于骨再生和 IL-1β 及 IL-10 水平的提高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Photodiagnosis and Photodynamic Therapy ONCOLOGY-

CiteScore

5.80

自引率

24.20%

发文量

509

审稿时长

50 days

期刊介绍： Photodiagnosis and Photodynamic Therapy is an international journal for the dissemination of scientific knowledge and clinical developments of Photodiagnosis and Photodynamic Therapy in all medical specialties. The journal publishes original articles, review articles, case presentations, "how-to-do-it" articles, Letters to the Editor, short communications and relevant images with short descriptions. All submitted material is subject to a strict peer-review process.