Investigation of the concentration and isotopic composition of Cu, Fe and Zn in human biofluids in the context of Alzheimer’s disease via tandem and multi-collector inductively coupled plasma-mass spectrometry

IF 3.6 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Trace Elements in Medicine and Biology Pub Date : 2024-08-30 DOI:10.1016/j.jtemb.2024.127515

Kasper Hobin , Lana Abou-Zeid , Iker Basabe Mendizabal , Tineke Van Vrekhem , Marijke Miatton , Bregje D’Haeze , Marta Scarioni , Tim Van Langenhove , Frank Vanhaecke

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引用次数: 0

Abstract

Studies on essential trace elements in the context of Alzheimer’s disease (AD) concluded that Cu, Fe and Zn interact with amyloid-β, accelerating plaque formation in the brain. Additionally, Cu and Fe in the vicinity of plaques produce reactive oxygen species (ROS) resulting in oxidative stress, whereas Zn plays a role in the antioxidant defence as a co-factor for antioxidants. In this work, the Cu, Fe and Zn concentrations and isotope ratios were determined in whole blood, blood serum and cerebrospinal fluid of 10 patients diagnosed with AD and 8 control individuals, using tandem (ICP-MS/MS) and multi-collector inductively coupled plasma-mass spectrometry (MC-ICP-MS), respectively. In whole blood and blood serum of AD patients, a heavier Cu isotopic composition was observed (significant for whole blood only) compared to controls. Albumin levels in cerebrospinal fluid tend to increase with age, which could indicate an increased leakiness of the blood-brain barrier. In cerebrospinal fluid, a large variability was observed for the Cu and Fe isotope ratios, potentially resulting from that leakiness at the blood-brain barrier. Therefore, potential effects of AD on the concentration and isotopic composition of essential elements in cerebrospinal fluid related to amyloid-β formation could be hidden. Finally, in blood serum, Zn, urea and creatinine concentrations showed an increase with age and showed a significant difference between sexes.

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通过串联和多收集器电感耦合等离子体质谱法研究阿尔茨海默病背景下人体生物流体中铜、铁和锌的浓度和同位素组成。

关于阿尔茨海默病（AD）中必需微量元素的研究认为，铜、铁和锌与淀粉样蛋白-β相互作用，加速大脑中斑块的形成。此外，斑块附近的铜和铁会产生活性氧（ROS），导致氧化应激，而锌则作为抗氧化剂的辅助因子在抗氧化防御中发挥作用。本研究采用串联（ICP-MS/MS）和多收集器电感耦合等离子体质谱法（MC-ICP-MS）分别测定了 10 例 AD 患者和 8 例对照者的全血、血清和脑脊液中铜、铁和锌的浓度及同位素比值。与对照组相比，在注意力缺失症患者的全血和血清中观察到较重的铜同位素组成（仅在全血中具有显著性）。脑脊液中的白蛋白水平随着年龄的增长而增加，这可能表明血脑屏障的渗漏性增加。在脑脊液中，观察到铜和铁同位素比值变化很大，这可能是血脑屏障渗漏造成的。因此，AD对脑脊液中与淀粉样β形成有关的必需元素的浓度和同位素组成的潜在影响可能是隐性的。最后，血清中锌、尿素和肌酐的浓度随着年龄的增长而增加，并且在性别之间存在显著差异。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Trace Elements in Medicine and Biology 医学-内分泌学与代谢

CiteScore

6.60

自引率

2.90%

发文量

202

审稿时长

85 days

期刊介绍： The journal provides the reader with a thorough description of theoretical and applied aspects of trace elements in medicine and biology and is devoted to the advancement of scientific knowledge about trace elements and trace element species. Trace elements play essential roles in the maintenance of physiological processes. During the last decades there has been a great deal of scientific investigation about the function and binding of trace elements. The Journal of Trace Elements in Medicine and Biology focuses on the description and dissemination of scientific results concerning the role of trace elements with respect to their mode of action in health and disease and nutritional importance. Progress in the knowledge of the biological role of trace elements depends, however, on advances in trace elements chemistry. Thus the Journal of Trace Elements in Medicine and Biology will include only those papers that base their results on proven analytical methods. Also, we only publish those articles in which the quality assurance regarding the execution of experiments and achievement of results is guaranteed.