3D printing of strontium-enriched biphasic calcium phosphate scaffolds for bone regeneration

IF 3.3 2区 医学 Q2 ENGINEERING, BIOMEDICAL
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引用次数: 0

Abstract

Calcium phosphate (CaP) scaffolds doping with therapeutic ions are one of the focuses of recent bone tissue engineering research. Among the therapeutic ions, strontium stands out for its role in bone remodeling. This work reports a simple method to produce Sr-doped 3D-printed CaP scaffolds, using Sr-doping to induce partial phase transformation from β-tricalcium phosphate (β-TCP) to hydroxyapatite (HA), resulting in a doped biphasic calcium phosphate (BCP) scaffold. Strontium carbonate (SrCO3) was incorporated in the formulation of the 3D-printing ink, studying β-TCP:SrO mass ratios of 100:0, 95:5, and 90:10 (named as β-TCP, β-TCP/5-Sr, and β-TCP/10-Sr, respectively). Adding SrCO3 in the 3D-printing ink led to a slight increase in viscosity but did not affect its printability, resulting in scaffolds with a high printing fidelity compared to the computational design. Interestingly, Sr was incorporated into the lattice structure of the scaffolds, forming hydroxyapatite (HA). No residual SrO or SrCO3 were observed in the XRD patterns of any composition, and HA was the majority phase of the β-TCP/10-Sr scaffolds. The addition of Sr increased the compression strength of the scaffolds, with both β-TCP/5-Sr and β-TCP/10-Sr performing better than the β-TCP. Overall, β-TCP/5-Sr presented higher mineralized nodules and mechanical strength, while β-TCP scaffolds presented superior cell viability. The incorporation of SrCO3 in the ink formulation is a viable method to obtain Sr-BCP scaffolds. Thus, this approach could be explored with other CaP scaffolds aiming to optimize their performance and the addition of alternative therapeutic ions.

Abstract Image

用于骨再生的富锶双相磷酸钙支架的三维打印技术。
掺入治疗离子的磷酸钙(CaP)支架是近年来骨组织工程研究的重点之一。在治疗离子中,锶因其在骨重塑中的作用而脱颖而出。这项工作报告了一种生产掺锶三维打印 CaP 支架的简单方法,利用掺锶诱导从β-磷酸三钙(β-TCP)到羟基磷灰石(HA)的部分相变,形成掺锶双相磷酸钙(BCP)支架。在三维打印墨水的配方中加入了碳酸锶(SrCO3),研究的β-TCP与SrO的质量比分别为100:0、95:5和90:10(分别命名为β-TCP、β-TCP/5-Sr和β-TCP/10-Sr)。在三维打印墨水中加入 SrCO3 会导致粘度略有增加,但不会影响其打印性能,因此与计算设计相比,支架的打印保真度较高。有趣的是,锶融入了支架的晶格结构,形成了羟基磷灰石(HA)。在任何成分的 XRD 图谱中都没有观察到残留的 SrO 或 SrCO3,而且 HA 是 β-TCP/10-Sr 支架的主要相。添加 Sr 增加了支架的压缩强度,β-TCP/5-Sr 和 β-TCP/10-Sr 的压缩强度均优于 β-TCP。总体而言,β-TCP/5-Sr 具有更高的矿化结核和机械强度,而 β-TCP 支架则具有更高的细胞存活率。在油墨配方中加入 SrCO3 是获得 Sr-BCP 支架的可行方法。因此,这种方法可用于其他 CaP 支架,以优化其性能和添加替代治疗离子。
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来源期刊
Journal of the Mechanical Behavior of Biomedical Materials
Journal of the Mechanical Behavior of Biomedical Materials 工程技术-材料科学:生物材料
CiteScore
7.20
自引率
7.70%
发文量
505
审稿时长
46 days
期刊介绍: The Journal of the Mechanical Behavior of Biomedical Materials is concerned with the mechanical deformation, damage and failure under applied forces, of biological material (at the tissue, cellular and molecular levels) and of biomaterials, i.e. those materials which are designed to mimic or replace biological materials. The primary focus of the journal is the synthesis of materials science, biology, and medical and dental science. Reports of fundamental scientific investigations are welcome, as are articles concerned with the practical application of materials in medical devices. Both experimental and theoretical work is of interest; theoretical papers will normally include comparison of predictions with experimental data, though we recognize that this may not always be appropriate. The journal also publishes technical notes concerned with emerging experimental or theoretical techniques, letters to the editor and, by invitation, review articles and papers describing existing techniques for the benefit of an interdisciplinary readership.
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