Genome-wide identification of cannabinoid biosynthesis genes in non-drug type Cannabis (Cannabis sativa L.) cultivar.

IF 4.1 Q1 PHARMACOLOGY & PHARMACY
Benny Jian Rong Sng, Yu Jeong Jeong, Sing Hui Leong, Jae Cheol Jeong, Jiyoung Lee, Sarojam Rajani, Cha Young Kim, In-Cheol Jang
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Abstract

Background: Cannabis sativa cultivars can be classified as marijuana or hemp, depending on its amount of the psychoactive cannabinoid Δ9-tetrahydrocannabinolic acid (THCA). Hemp Cheungsam is a non-drug type Cannabis sativa that is characterized by low THCA content. However, the transcripts and expression profile of cannabinoid biosynthesis pathway genes of hemp Cheungsam have not been investigated.

Methods: RNA-sequencing (RNA-seq) was performed on three different tissue types (flower, leaf, and stem) of hemp Cheungsam to understand their transcriptomes. The expression of cannabinoid biosynthesis pathway genes was further analyzed in each tissue type. Multiple sequence alignment and conserved domain analyses were used to investigate the homologs of cannbinoid biosynthesis genes.

Results: We found that the cannabinoid biosynthesis pathway was mainly expressed in the flowers of hemp Cheungsam, similar to other Cannabis cultivars. However, expression of cannabidiolic acid (CBDA) synthase was much higher than THCA synthase and cannabichromenic acid (CBCA) synthase, suggesting that the transcription profile favors CBDA biosynthesis. Sequence analysis of cannabinoid biosynthesis pathway genes suggested the identity of orthologs in hemp Cheungsam.

Conclusions: Cannabinoid biosynthesis in hemp Cheungsam mostly occurs in the flowers, compared to other plant organs. While CBDA synthase expression is high, THCA and CBCA synthase expression is considerably low, indicating lesser THCA biosynthesis and thus low THCA content. Sequence analysis of key genes (CBDA, THCA, and CBCA synthases) of the cannabinoid biosynthetic pathway indicates that orthologs are present in hemp Cheungsam.

非药物型大麻(Cannabis sativa L.)栽培品种中大麻素生物合成基因的全基因组鉴定。
背景:根据精神活性大麻素Δ9-四氢大麻酚酸(THCA)的含量,大麻栽培品种可分为大麻(marijuana)和大麻(hahp)。大麻祥三是一种非药物型大麻,其特点是四氢大麻酚含量较低。然而,人们尚未研究过大麻樟的大麻素生物合成途径基因的转录本和表达谱:方法:对长生麻的三种不同组织类型(花、叶和茎)进行了 RNA 测序(RNA-seq),以了解其转录组。进一步分析了每种组织类型中大麻素生物合成途径基因的表达情况。多重序列比对和保守结构域分析用于研究大麻素生物合成基因的同源物:结果:我们发现大麻生物合成途径主要在大麻 Cheungsam 的花中表达,这与其他大麻栽培品种相似。然而,大麻二酸(CBDA)合成酶的表达量远高于四氢大麻酚合成酶和大麻色酸(CBCA)合成酶,这表明转录特征更倾向于 CBDA 的生物合成。大麻素生物合成途径基因的序列分析表明,大麻祥三中存在同源基因:结论:与其他植物器官相比,Cheungsam 大麻中的大麻素生物合成主要发生在花中。虽然 CBDA 合成酶的表达量很高,但 THCA 和 CBCA 合成酶的表达量却相当低,这表明 THCA 的生物合成较少,因此 THCA 的含量也较低。对大麻素生物合成途径的关键基因(CBDA、THCA 和 CBCA 合成酶)进行的序列分析表明,Cheungsam 大麻中存在同源基因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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