Impact of water temperature on oxidative stress and intestinal microbiota in pearl-spot chromis, Chromis notata (Temminck & Schlegel, 1843)

IF 1.8 3区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Jin A Kim , Young-Su Park , Jun-Hwan Kim , Cheol Young Choi
{"title":"Impact of water temperature on oxidative stress and intestinal microbiota in pearl-spot chromis, Chromis notata (Temminck & Schlegel, 1843)","authors":"Jin A Kim ,&nbsp;Young-Su Park ,&nbsp;Jun-Hwan Kim ,&nbsp;Cheol Young Choi","doi":"10.1016/j.cbpb.2024.111029","DOIUrl":null,"url":null,"abstract":"<div><p>Water temperature is an abiotic factor influencing fish metabolism and physiological responses. As poikilothermic creatures, fish are notable sensitivity to fluctuations in water temperature, which also significantly influences intestinal microbial proliferation. This study aimed to investigate the impact of both low (8 °C) and high (28 °C) water temperatures on oxidative stress and the intestinal microbiota of <em>Chromis notata</em>, a species that has recently migrated northward owing to changes in sea water temperature. Laboratory experiments were conducted to assess changes in superoxide dismutase (SOD), catalase (CAT), and lysozyme activities, as well as changes in the abundance and diversity of intestinal microbiota. The activities of antioxidant enzymes, specifically SOD and CAT, in <em>C. notata</em> exposed to low and high temperatures, showed an increase compared to the control group (maintained at 18 °C). Moreover, liver H<sub>2</sub>O<sub>2</sub> levels exhibited a significant increase over time. Conversely, plasma lysozyme activity significantly decreased in groups subjected to low and high water temperatures compared to the control group. Analyzing changes in the intestinal microbiota, we observed an increase in the proportion of Firmicutes but a decrease in Proteobacteria, which are known for their role in immune enhancement, in <em>C. notata</em> exposed to both low and high water temperatures. We propose that alterations in water temperature impact the antioxidant enzyme activity of <em>C. notata</em>, leading to compromised immune responses and disruption of the biological balance of the intestinal microbiota, potentially affecting the host's survival.</p></div>","PeriodicalId":55236,"journal":{"name":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","volume":"275 ","pages":"Article 111029"},"PeriodicalIF":1.8000,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Comparative Biochemistry and Physiology B-Biochemistry & Molecular Biology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1096495924000964","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Water temperature is an abiotic factor influencing fish metabolism and physiological responses. As poikilothermic creatures, fish are notable sensitivity to fluctuations in water temperature, which also significantly influences intestinal microbial proliferation. This study aimed to investigate the impact of both low (8 °C) and high (28 °C) water temperatures on oxidative stress and the intestinal microbiota of Chromis notata, a species that has recently migrated northward owing to changes in sea water temperature. Laboratory experiments were conducted to assess changes in superoxide dismutase (SOD), catalase (CAT), and lysozyme activities, as well as changes in the abundance and diversity of intestinal microbiota. The activities of antioxidant enzymes, specifically SOD and CAT, in C. notata exposed to low and high temperatures, showed an increase compared to the control group (maintained at 18 °C). Moreover, liver H2O2 levels exhibited a significant increase over time. Conversely, plasma lysozyme activity significantly decreased in groups subjected to low and high water temperatures compared to the control group. Analyzing changes in the intestinal microbiota, we observed an increase in the proportion of Firmicutes but a decrease in Proteobacteria, which are known for their role in immune enhancement, in C. notata exposed to both low and high water temperatures. We propose that alterations in water temperature impact the antioxidant enzyme activity of C. notata, leading to compromised immune responses and disruption of the biological balance of the intestinal microbiota, potentially affecting the host's survival.

Abstract Image

水温对珠点虹鳟氧化应激和肠道微生物群的影响
水温是影响鱼类新陈代谢和生理反应的非生物因素。鱼类作为变温动物,对水温波动表现出明显的敏感性,而水温波动也会显著影响肠道微生物的增殖。本研究旨在调查低水温(8 °C)和高水温(28 °C)对鲑鱼氧化应激和肠道微生物群的影响。实验室实验评估了超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和溶菌酶活性的变化,以及肠道微生物群丰度和多样性的变化。与对照组(保持在 18 °C)相比,暴露于低温和高温下的 C. notata 的抗氧化酶(特别是 SOD 和 CAT)活性有所提高。此外,肝脏中的 H2O2 含量也随着时间的推移而显著增加。相反,与对照组相比,低水温组和高温组的血浆溶菌酶活性显著降低。在分析肠道微生物群的变化时,我们观察到,在暴露于低水温和高水温的 C. notata 中,固着菌的比例增加,而蛋白质细菌的比例下降,而蛋白质细菌在免疫增强中的作用是众所周知的。我们认为,水温的变化会影响 C. notata 的抗氧化酶活性,导致免疫反应受损和肠道微生物群的生物平衡被破坏,从而可能影响宿主的生存。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
4.60
自引率
4.50%
发文量
77
审稿时长
22 days
期刊介绍: Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part B: Biochemical and Molecular Biology (CBPB), focuses on biochemical physiology, primarily bioenergetics/energy metabolism, cell biology, cellular stress responses, enzymology, intermediary metabolism, macromolecular structure and function, gene regulation, evolutionary genetics. Most studies focus on biochemical or molecular analyses that have clear ramifications for physiological processes.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信