PPR21 is involved in the splicing of nad2 introns via interacting with PPR-small MutS-related 1 and small PPR protein 2 and is essential to maize seed development.

IF 6.6 2区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yan-Zhuo Yang, Xin-Yuan Liu, Song Gao, Shu-Guang Zhang, Bao-Cai Tan
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引用次数: 0

Abstract

Pentatricopeptide repeat (PPR) proteins are a large group of eukaryote-specific RNA-binding proteins that play pivotal roles in plant organelle gene expression. Here, we report the function of PPR21 in mitochondrial intron splicing and its role in maize kernel development. PPR21 is a typical P-type PPR protein targeted to mitochondria. The ppr21 mutants are arrested in embryogenesis and endosperm development, leading to embryo lethality. Null mutations of PPR21 reduce the splicing efficiency of nad2 intron 1, 2, and 4 and impair the assembly and activity of mitochondrial complex I. Previous studies show that the P-type PPR protein EMP12 is required for the splicing of identical introns. However, our protein interaction analyses reveal that PPR21 does not interact with EMP12. Instead, both PPR21 and EMP12 interact with the small MutS-related (SMR) domain-containing PPR protein 1 (PPR-SMR1) and the short P-type PPR protein 2 (SPR2). PPR-SMR1 interacts with SPR2, and both proteins are required for the splicing of many introns in mitochondria, including nad2 intron 1, 2, and 4. These results suggest that a PPR21-(PPR-SMR1/SPR2)-EMP12 complex is involved in the splicing of nad2 introns in maize mitochondria.

PPR21 通过与 PPR-SMR1 和 SPR2 相互作用参与 nad2 内含子的剪接,对玉米种子的发育至关重要。
五肽重复(PPR)蛋白是一大类真核生物特异性 RNA 结合蛋白,在植物细胞器基因表达中发挥着关键作用。在这里,我们报告了 PPR21 在线粒体内含子剪接中的功能及其在玉米籽粒发育中的作用。PPR21 是一种典型的以线粒体为靶标的 P 型 PPR 蛋白。ppr21突变体在胚胎发生和胚乳发育过程中停滞,导致胚胎死亡。PPR21 的无效突变会降低 nad2 内含子 1、2 和 4 的剪接效率,并损害线粒体复合体 I 的组装和活性。以前的研究表明,P 型 PPR 蛋白 EMP12 是相同内含子剪接所必需的。然而,我们的蛋白质相互作用分析表明,PPR21 并不与 EMP12 相互作用。相反,PPR21 和 EMP12 都与含小突变相关(SMR)结构域的 PPR 蛋白 1(PPR-SMR1)和短 P 型 PPR 蛋白 2(SPR2)相互作用。PPR-SMR1 与 SPR2 相互作用,线粒体中许多内含子的剪接都需要这两种蛋白,包括 nad2 内含子 1、2 和 4。这些结果表明,PPR21-(PPR-SMR1/SPR2)-EMP12 复合物参与了玉米线粒体中 nad2 内含子的剪接。
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来源期刊
Journal of Genetics and Genomics
Journal of Genetics and Genomics 生物-生化与分子生物学
CiteScore
8.20
自引率
3.40%
发文量
4756
审稿时长
14 days
期刊介绍: The Journal of Genetics and Genomics (JGG, formerly known as Acta Genetica Sinica ) is an international journal publishing peer-reviewed articles of novel and significant discoveries in the fields of genetics and genomics. Topics of particular interest include but are not limited to molecular genetics, developmental genetics, cytogenetics, epigenetics, medical genetics, population and evolutionary genetics, genomics and functional genomics as well as bioinformatics and computational biology.
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