Collagen and collagenases in mare’s endometrium with endometrosis

IF 2.4 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY
Luiz Augusto M. Centeno , Henrique B.A. Bastos , Verônica L.C. Bueno , Janislene M. Trentin , MarianiF. Fiorenza , Welden Panziera , Gustavo H.Z. Winter , Nelson A. Kretzmann , Sandra Fiala-Rechsteiner , Rodrigo C. Mattos , Mara I.B. Rubin
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Abstract

Equine endometrosis is a degenerative and predominantly fibrotic condition resulting from progressive and irreversible multifactorial causes that influence the endometrium of mare. Tissue remodeling in the equine endometrium occurs as part of the pathogenesis of endometrosis, a process characterized by a shift in extracellular matrix (ECM) components. The relationship between matrix metalloproteinases and their specific inhibitors is crucial for the remodeling process. Collagen play a significant role in maintaining a healthy uterus and may promote fibrotic processes. The aim of this study was to quantify endometrial collagen deposition using picrosirius 25 red (PSR) staining, and to evaluate gene expression of collagen type 2 (COL-2) and 3 (COL-3), matrix metalloproteinases 1 (MMP-1) and 2 (MMP-2), their tissue inhibitor (TIMP-2), and tumor necrosis factor (TNF-α) in the endometrium of mares with different grades of fibrosis. The samples (n = 34) were classified into three categories based on the frequency and distribution of fibrosis-related changes in the endometrium: Category I (healthy endometrium, n = 12), Category II (moderate fibrosis, n = 12), and Category III (severe fibrosis, n = 10). Collagen quantification demonstrate a substantial proportional increase (P < 0.0001) in collagen deposition across Category I (11.72 ± 1.39 %), Category II (17.76 ± 1.29 %), and Category III (24.15 ± 1.87 %). In transcript evaluations, higher COL-2 expression was found in Category II than in mares classified as Category I or III. MMP-1 showed increased transcript expression in Category II compared to Category III endometrial samples. Higher expression of MMP-2 was detected in Category III than in Category I and II. TIMP-2 showed lower mRNA expression in Category III vs Category I and II. However, TNF-α gene expression was higher in Category II than in Categories I and III. This study demonstrates that endometrial evaluation using PSR can play an important role in routine analyses for the detection and objective quantification of collagen in endometrial tissues. Additionally, this study demonstrated through gene expression analysis that MMP-1 may be linked to physiological endometrial remodeling. In contrast, MMP-2 could be associated with fibrogenesis in the endometrium, which is regulated by the inhibitor TIMP-2. Furthermore, COL-2 and TNF-α could be considered as biological markers involved in the progression endometrosis in mares. As such, the results of this study may contribute to the development of future antifibrotic therapies that aim to delay or even reverse the pathological remodeling of the extracellular matrix in the uterus, in addition to optimizing the diagnosis and prognosis of endometrial fibrosis in mares.

患有子宫内膜炎的母马子宫内膜中的胶原蛋白和胶原酶。
马子宫内膜病变是一种退化性疾病,主要是由于影响母马子宫内膜的渐进性和不可逆的多因素原因导致的纤维化。马子宫内膜的组织重塑是子宫内膜病变发病机制的一部分,这一过程的特点是细胞外基质(ECM)成分发生变化。基质金属蛋白酶及其特异性抑制剂之间的关系对重塑过程至关重要。胶原蛋白在维持子宫健康方面发挥着重要作用,同时也可能促进子宫纤维化过程。本研究的目的是使用皮氏25红(PSR)染色法量化子宫内膜胶原沉积,并评估不同纤维化程度的母马子宫内膜中2型胶原(COL-2)和3型胶原(COL-3)、基质金属蛋白酶1(MMP-1)和2(MMP-2)、其组织抑制剂(TIMP-2)以及肿瘤坏死因子(TNF-α)的基因表达。根据子宫内膜纤维化相关变化的频率和分布情况,将样本(n = 34)分为三类:I类(健康子宫内膜,n = 12)、II类(中度纤维化,n = 12)和III类(重度纤维化,n = 10)。胶原蛋白定量显示,子宫内膜中的胶原蛋白比例大幅增加(P
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来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
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