Symbiont-mediated antisense RNA delivery controls Nosema ceranae infections in Apis mellifera

IF 3.6 3区生物学 Q1 ZOOLOGY

Journal of invertebrate pathology Pub Date : 2024-09-05 DOI:10.1016/j.jip.2024.108185

Sihan Wang , Haoyang Li , Ruyi Shi , Yuqi Fu

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Abstract

Nosema ceranae is a main parasite for honeybees (Apis mellifera) which causes colony collapse in spring. Effective management of N. ceranae infections in bees is imperative for beekeepers. RNA interference (RNAi) has been proven a promising method to control bee pathogens, including IAPV, Varroa destructor, and Nosema. Most studies in this field focused on oral inoculation of double-stranded RNA (dsRNA). We developed an easier method with long-term RNAi effects by engineering the bee symbiont, Bacillus subtilis, to deliver single-stranded antisense RNA (asRNA) in the bee guts, targeting N. ceranae genes. We interfered with the expression of a spore wall protein (SWP12) and a polar tube protein (PTP3) of N. ceranae, resulting in a 60.5% increase in bee lifespan and a 72.7% decrease in Nosema spore load. Our research introduced a novel approach to bee parasite control: B. subtilis-mediated asRNA delivery. Our strategy simplifies the procedure of RNAi, presenting a more efficient mechanism with both prophylactic and therapeutic effects on N. ceranae-infected bees.

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共生体介导的反义 RNA 递送可控制蜜蜂中的陶瓷鼻疽瘤病毒感染。

陶瓷鼻疽酵母菌是蜜蜂（Apis mellifera）的主要寄生虫，春季会导致蜂群崩溃。对养蜂人来说，有效控制蜜蜂中的神经荚膜瘤病毒感染势在必行。RNA 干扰（RNAi）已被证明是控制蜜蜂病原体（包括 IAPV、Varroa destructor 和 Nosema）的有效方法。该领域的大多数研究都集中于双链 RNA（dsRNA）的口服接种。我们通过改造蜜蜂共生体枯草芽孢杆菌（Bacillus subtilis），在蜜蜂内脏中递送单链反义 RNA（asRNA），靶向 N. ceranae 基因，从而开发出一种更简便且具有长期 RNAi 效果的方法。我们干扰了N. ceranae的孢子壁蛋白（SWP12）和极管蛋白（PTP3）的表达，结果蜜蜂的寿命延长了60.5%，Nosema孢子量减少了72.7%。我们的研究为蜜蜂寄生虫控制引入了一种新方法：枯草杆菌介导的 asRNA 递送。我们的策略简化了 RNAi 的过程，提供了一种更有效的机制，对感染 N. ceranae 的蜜蜂具有预防和治疗作用。

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来源期刊

Journal of invertebrate pathology 生物-动物学

CiteScore

6.10

自引率

5.90%

发文量

审稿时长

1 months

期刊介绍： The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.