Whole-genome sequence analysis of morphological changes in Haemophilus influenzae after beta-lactam exposure

IF 3.7 3区医学 Q2 INFECTIOUS DISEASES

Journal of global antimicrobial resistance Pub Date : 2024-09-06 DOI:10.1016/j.jgar.2024.08.001

Lucia Malisova , Vladislav Jakubu , Ibrahim Bitar , Daniel Krsek , Helena Zemlickova

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引用次数: 0

Abstract

This report describes mutations in genes responsible for cell deformities in haemophili under beta-lactam pressure in vitro. Light and transmission electron microscopy confirmed a hypothesis regarding changes in the shape of haemophili that had become more filamentous in the presence of ampicillin (2 mg/L) and cefuroxime (8 mg/L) after 30 days of serial passage. Short-axis size increased by 28% (from 0.767 to 1.06 µm) and long-axis length increased by 54% (from 1 to 2.175 µm). Additionally, whole-genome sequencing analysis (Illumina platform, software PROKKA) revealed a variety of mutations in genes responsible for cell morphology in isolates examined in this study: ftsI (A1576 → C; G1154 → C; T986 → C; G1684 → C), mreB (C476 → T), mreC (A5 → G), mrdA (A1148 → G; C179 → T; G1613 → T), mrdB (T668 → G), mltC (C1016 → T) and rodA (T668 → G). The results of this study indicate that shifts in bacterial shape could play a role in the adaptation of haemophili to a new niche created by beta-lactams as a strategy of antibiotic therapy survival.

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对接触β-内酰胺后流感嗜血杆菌形态变化的全基因组序列分析。

本报告描述了在体外β-内酰胺类药物的压力下，导致嗜血杆菌细胞畸形的基因突变。光镜和透射电子显微镜证实了一种假设，即在氨苄西林（2 毫克/升）和头孢呋辛（8 毫克/升）的作用下，嗜血杆菌的形状在连续通过 30 天后会发生变化，变得更像丝状。短轴的尺寸增加了 28%（从 0.767μm 增加到 1.06μm），长轴的长度增加了 54%（从 1μm 增加到 2.175μm）。此外，全基因组测序分析（Illumina 平台，软件 PROKKA）显示，本研究中检测的分离株中负责细胞形态的基因发生了多种突变：ftsI（A1576 → C；G1154 → C；T986 → C；G1684 → C）、mreB（C476 → T）、mreC（A5 → G）、mrdA（A1148 → G；C179 → T；G1613 → T）、mrdB（T668 → G）、mltC（C1016 → T）、rodA（T668 → G）。这项研究的结果表明，细菌形状的改变可能是嗜血杆菌适应β-内酰胺类药物创造的新生态位的一种策略。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY

CiteScore

8.70

自引率

2.20%

发文量

285

审稿时长

34 weeks

期刊介绍： The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.