Association of Borrelia burgdorferi with nuchal bursitis and elevated outer surface protein A-specific serum antibodies in horses of the northeastern United States.
Erin K Pearson, Cassandra Guarino, Marta Cercone, Thomas Divers, Jenna Lambert, José García-López, Amy L Johnson, Julie B Engiles, Richard Marconi, Jade Smith, Kara Brown, Toby Pinn-Woodcock
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引用次数: 0
Abstract
Objective: Evaluate the incidence of Borrelia burgdorferi in cases of equine nuchal bursitis (NB) and investigate the relationship between elevated serum outer surface protein A (OspA) antibodies and the molecular identification of B burgdorferi in bursal tissue or synovial fluid. Additionally, describe clinical cases and compare the histologic changes in NB with and without detection of B burgdorferi.
Methods: This was a retrospective multicenter cohort study (2013 to 2022). Medical records from horses with a diagnosis of NB and B burgdorferi PCR testing on NB tissue or synovial fluid were reviewed. The study population included 11 horses with a postmortem diagnosis of NB, 19 horses from the northeastern US with an antemortem diagnosis of B burgdorferi PCR-positive NB, and 15 healthy controls without evidence of NB and unvaccinated for B burgdorferi. Where serum was available, Lyme multiplex assay results were compared with controls and ELISAs targeting individual B burgdorferi antigens were performed. Histologic findings in nuchal bursa tissue were compared between NB cases with and without B burgdorferi PCR detection.
Results: Serum OspA antibody values in B burgdorferi-positive NB cases (n = 13) were significantly elevated (P < .001) compared to controls (15), and OspA was the predominant antigen detected by ELISA (8). Histopathology did not vary between NB cases with (n = 9) and without (6) B burgdorferi PCR detection.
Conclusions: The presence of B burgdorferi in the nuchal bursa of horses is associated with increased serum OspA antibodies.
Clinical relevance: The role of B burgdorferi in equine NB may be underestimated, and targeted therapy requires investigation.
目的评估马颈滑囊炎(NB)病例中布氏包柔氏菌的发病率,研究血清外表面蛋白A(OspA)抗体升高与滑囊组织或滑液中布氏包柔氏菌分子鉴定之间的关系。此外,还要描述临床病例,并比较检测到和未检测到布氏杆菌的 NB 组织学变化:这是一项回顾性多中心队列研究(2013 年至 2022 年)。方法:这是一项回顾性多中心队列研究(2013 年至 2022 年)。研究人员查阅了确诊为 NB 且在 NB 组织或滑液中进行了 B burgdorferi PCR 检测的马匹的医疗记录。研究对象包括 11 匹尸检诊断为 NB 的马、19 匹来自美国东北部的尸检诊断为 B burgdorferi PCR 阳性 NB 的马、15 匹无 NB 病例且未接种 B burgdorferi 疫苗的健康对照组。在有血清的情况下,莱姆多重检测结果与对照组进行了比较,并进行了针对个别布氏菌抗原的酶联免疫吸附试验。对检测到和未检测到布氏杆菌的 NB 病例的颈静脉滑囊组织的组织学结果进行了比较:结果:与对照组(15 例)相比,布氏杆菌阳性 NB 病例(n = 13 例)的血清 OspA 抗体值明显升高(P < .001),ELISA 检测到的主要抗原是 OspA(8 例)。有(9 例)和没有(6 例)检测到 B burgdorferi PCR 的 NB 病例在组织病理学上没有差异:结论:马的颈囊中存在布氏菌与血清 OspA 抗体的增加有关:临床意义:布氏杆菌在马NB中的作用可能被低估,需要研究有针对性的治疗方法。
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