Association of Borrelia burgdorferi with nuchal bursitis and elevated outer surface protein A-specific serum antibodies in horses of the northeastern United States.

IF 1.6 2区 农林科学 Q2 VETERINARY SCIENCES
Erin K Pearson, Cassandra Guarino, Marta Cercone, Thomas Divers, Jenna Lambert, José García-López, Amy L Johnson, Julie B Engiles, Richard Marconi, Jade Smith, Kara Brown, Toby Pinn-Woodcock
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引用次数: 0

Abstract

Objective: Evaluate the incidence of Borrelia burgdorferi in cases of equine nuchal bursitis (NB) and investigate the relationship between elevated serum outer surface protein A (OspA) antibodies and the molecular identification of B burgdorferi in bursal tissue or synovial fluid. Additionally, describe clinical cases and compare the histologic changes in NB with and without detection of B burgdorferi.

Methods: This was a retrospective multicenter cohort study (2013 to 2022). Medical records from horses with a diagnosis of NB and B burgdorferi PCR testing on NB tissue or synovial fluid were reviewed. The study population included 11 horses with a postmortem diagnosis of NB, 19 horses from the northeastern US with an antemortem diagnosis of B burgdorferi PCR-positive NB, and 15 healthy controls without evidence of NB and unvaccinated for B burgdorferi. Where serum was available, Lyme multiplex assay results were compared with controls and ELISAs targeting individual B burgdorferi antigens were performed. Histologic findings in nuchal bursa tissue were compared between NB cases with and without B burgdorferi PCR detection.

Results: Serum OspA antibody values in B burgdorferi-positive NB cases (n = 13) were significantly elevated (P < .001) compared to controls (15), and OspA was the predominant antigen detected by ELISA (8). Histopathology did not vary between NB cases with (n = 9) and without (6) B burgdorferi PCR detection.

Conclusions: The presence of B burgdorferi in the nuchal bursa of horses is associated with increased serum OspA antibodies.

Clinical relevance: The role of B burgdorferi in equine NB may be underestimated, and targeted therapy requires investigation.

美国东北部马匹的布氏杆菌与颈囊炎和外表面蛋白A特异性血清抗体升高的关系。
目的评估马颈滑囊炎(NB)病例中布氏包柔氏菌的发病率,研究血清外表面蛋白A(OspA)抗体升高与滑囊组织或滑液中布氏包柔氏菌分子鉴定之间的关系。此外,还要描述临床病例,并比较检测到和未检测到布氏杆菌的 NB 组织学变化:这是一项回顾性多中心队列研究(2013 年至 2022 年)。方法:这是一项回顾性多中心队列研究(2013 年至 2022 年)。研究人员查阅了确诊为 NB 且在 NB 组织或滑液中进行了 B burgdorferi PCR 检测的马匹的医疗记录。研究对象包括 11 匹尸检诊断为 NB 的马、19 匹来自美国东北部的尸检诊断为 B burgdorferi PCR 阳性 NB 的马、15 匹无 NB 病例且未接种 B burgdorferi 疫苗的健康对照组。在有血清的情况下,莱姆多重检测结果与对照组进行了比较,并进行了针对个别布氏菌抗原的酶联免疫吸附试验。对检测到和未检测到布氏杆菌的 NB 病例的颈静脉滑囊组织的组织学结果进行了比较:结果:与对照组(15 例)相比,布氏杆菌阳性 NB 病例(n = 13 例)的血清 OspA 抗体值明显升高(P < .001),ELISA 检测到的主要抗原是 OspA(8 例)。有(9 例)和没有(6 例)检测到 B burgdorferi PCR 的 NB 病例在组织病理学上没有差异:结论:马的颈囊中存在布氏菌与血清 OspA 抗体的增加有关:临床意义:布氏杆菌在马NB中的作用可能被低估,需要研究有针对性的治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.60
自引率
15.80%
发文量
539
审稿时长
6-16 weeks
期刊介绍: Published twice monthly, this peer-reviewed, general scientific journal provides reports of clinical research, feature articles and regular columns of interest to veterinarians in private and public practice. The News and Classified Ad sections are posted online 10 days to two weeks before they are delivered in print.
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