An RNA aptamer photoelectrochemical biosensor based on the exciton energy transfer constructed for theophylline detection

IF 2.6 4区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Analytical biochemistry Pub Date : 2024-09-05 DOI:10.1016/j.ab.2024.115658

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Abstract

A novel photoelectrochemical (PEC) biosensor was developed incorporating a specifically designed RNA aptamer for the detection of theophylline (TP). This involved utilizing two nucleotide base aptamers with tailored sequences designed to target TP. The 3′ end of a single-stranded RNA sequence (5′-GGAUACCA–(CH₂)₆–SH-3′) and the 5′ end of a complementary stranded RNA sequence (5′–HS–(CH₂)₆-CCUUGGAAGCC-3′) were linked to gold nanoparticles (AuNPs) and CdS quantum dots (QDs), respectively. These two single-stranded RNAs (ssRNA) formed a double-stranded RNA (dsRNA) capable of recognizing TP. This major structural change altered the spacing between QDs and NPs, which signaled the presence and concentration of TP. TP was photoelectrochemical catalytic oxidation by the hole of CdS QDs under illumination, then anode photocurrent was generated. Due to the increase in surface impedance and the effect of exciton energy transfer (EET) between QDs and AuNPs, the photocurrent would undergo varying degrees of change. TP was detected by changes in photocurrent. PEC detection of TP was achieved in the range of 0.1 μM–200 μM. The detection limit was 0.033 μM. The method exhibited commendable reproducibility and remarkable selectivity. The biosensor was used to measure TP content in tea, beverages and blood samples, resulting in satisfactory recovery rates.

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一种基于激子能量转移的 RNA 合子光电化学生物传感器，用于检测茶碱。

我们开发了一种新型光电化学（PEC）生物传感器，其中包含一种专门设计的 RNA 合体，用于检测茶碱（TP）。这涉及到利用两个核苷酸碱基适配体，其特定序列是针对 TP 而设计的。单链 RNA 序列（5'-GGAUACCA-(CH2)6-SH-3'）的 3' 端和互补链 RNA 序列（5'-HS-(CH2)6-CCUUGGAAGCC-3'）的 5' 端分别与金纳米粒子（AuNPs）和 CdS 量子点（QDs）相连。这两种单链 RNA（ssRNA）形成了能够识别 TP 的双链 RNA（dsRNA）。这一重大结构变化改变了 QDs 和 NPs 之间的间距，从而显示出 TP 的存在和浓度。在光照下，TP 被 CdS QDs 的空穴光电催化氧化，然后产生阳极光电流。由于表面阻抗的增加以及 QDs 和 AuNPs 之间激子能量转移（EET）的影响，光电流会发生不同程度的变化。通过光电流的变化来检测 TP。PEC 检测 TP 的范围为 0.1 μM 至 200 μM。检测限为 0.033 μM。该方法具有良好的重现性和显著的选择性。该生物传感器用于测量茶叶、饮料和血液样本中的 TP 含量，回收率令人满意。

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来源期刊

Analytical biochemistry 生物-分析化学

CiteScore

5.70

自引率

0.00%

发文量

283

审稿时长

44 days

期刊介绍： The journal''s title Analytical Biochemistry: Methods in the Biological Sciences declares its broad scope: methods for the basic biological sciences that include biochemistry, molecular genetics, cell biology, proteomics, immunology, bioinformatics and wherever the frontiers of research take the field. The emphasis is on methods from the strictly analytical to the more preparative that would include novel approaches to protein purification as well as improvements in cell and organ culture. The actual techniques are equally inclusive ranging from aptamers to zymology. The journal has been particularly active in: -Analytical techniques for biological molecules- Aptamer selection and utilization- Biosensors- Chromatography- Cloning, sequencing and mutagenesis- Electrochemical methods- Electrophoresis- Enzyme characterization methods- Immunological approaches- Mass spectrometry of proteins and nucleic acids- Metabolomics- Nano level techniques- Optical spectroscopy in all its forms. The journal is reluctant to include most drug and strictly clinical studies as there are more suitable publication platforms for these types of papers.