A fluorescent probe for monitoring carboxylesterases in pulmonary cells under permissive hypercapnia condition

IF 1.8 4区 化学 Q3 CHEMISTRY, ANALYTICAL
Xiao-Ping Wu, Na Yang, Qing-Qing Liu, Zhong-Quan Zhu
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Abstract

Herein, by combining the benzofuranone-derived fluorophore and the carbamate recognition group, a fluorescent probe named BFO-CarE was developed for monitoring the carboxylesterase (CarE) level in pulmonary cells under the permissive hypercapnia condition. It showed a notable fluorescence response towards CarE at 570 nm under the excitation of 510 nm. The in-solution tests revealed the advantages of BFO-CarE including high sensitivity, high specificity, relatively rapid response, and high steadiness. It was also low-toxic upon the pulmonary cell lines. During the intracellular imaging in pulmonary cells, BFO-CarE achieved the monitoring of the CarE level in both inhibition and activation status. In particular, BFO-CarE realized the visualization of the affection of the permissive hypercapnia condition on the CarE level, which indicated the hypoxia tolerance of CarE. This work was informative for investigating the impact of hypoxia in pulmonary cells, and the corresponding anaesthesia-related approaches.

Graphical abstract

Abstract Image

在允许性高碳酸血症条件下监测肺细胞中羧酸酯酶的荧光探针。
在此,通过将苯并呋喃酮衍生荧光团与氨基甲酸酯识别基团相结合,开发出了一种名为 BFO-CarE 的荧光探针,用于在允许性高碳酸血症条件下监测肺细胞中羧酸酯酶(CarE)的水平。在 510 纳米的激发下,该探针在 570 纳米波长处对 CarE 显示出明显的荧光反应。溶液内测试表明,BFO-CarE 具有灵敏度高、特异性强、反应相对迅速和稳定性高等优点。它对肺细胞株的毒性也很低。在对肺细胞进行胞内成像时,BFO-CarE 可监测抑制和激活状态下的 CarE 水平。特别是,BFO-CarE 实现了允许性高碳酸血症条件对 CarE 水平影响的可视化,这表明了 CarE 的低氧耐受性。这项工作对研究缺氧对肺细胞的影响以及相应的麻醉相关方法具有参考价值。
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来源期刊
Analytical Sciences
Analytical Sciences 化学-分析化学
CiteScore
2.90
自引率
18.80%
发文量
232
审稿时长
1 months
期刊介绍: Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.
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