Traditionally used edible medicinal plants protect against rotenone induced toxicity in SH-SY5Y cells-a prospect for the development of herbal nutraceuticals

IF 4.4 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
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Abstract

Plants are good sources of pharmacologically active compounds. The present study aimed to examine the neuroprotective potentials of the methanol extracts of Salix tetrasperma Roxb. leaf (STME) and Plantago asiatica L. (PAME), two edibles medicinal plants of Manipur, India against neurotoxicity induced by rotenone in SH-SY5Y cells. Free radical quenching activities were evaluated by ABTS and DPPH assays. The cytotoxicity of rotenone and the neuronal survival were assessed by MTT assay and MAP2 expression analysis. DCF-DA, Rhodamine 123 (Rh-123), and DAPI measured the intracellular reactive oxygen species (ROS) levels, mitochondrial membrane potential (MMP), and apoptotic nuclei, respectively. Superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities were also assessed. LC-QTOF-MS analysis was performed for the identification of the compounds present in STME and PAME. The study showed that both the plant extracts (STME and PAME) showed antioxidant and neuroprotective capabilities in rotenone-induced neurotoxicity by preventing oxidative stress through the reduction of intracellular ROS levels and reversing the activities of GPx, SOD, and CAT caused by rotenone. Further, both plants prevented apoptotic cell death by normalizing the steady state of MMP and protecting nuclear DNA condensation. LC-QTOF-MS analysis shows the presence of known neuroprotective compounds like uridine and gabapentin in STME and PAME respectively. The two plants might be an important source of natural antioxidants and nutraceuticals with neuroprotective abilities. This could be investigated further to formulate herbal nutraceuticals for the treatment of neurodegenerative disease like Parkinson's disease.

Abstract Image

传统食用药用植物可防止鱼藤酮诱导的 SH-SY5Y 细胞毒性--中草药保健品的开发前景广阔。
植物是药理活性化合物的良好来源。本研究旨在研究印度曼尼普尔的两种食用药用植物 Salix tetrasperma Roxb. 叶(STME)和 Plantago asiatica L. (PAME)的甲醇提取物对 SH-SY5Y 细胞由鱼藤酮诱导的神经毒性的保护潜力。通过 ABTS 和 DPPH 试验评估了自由基淬灭活性。通过 MTT 试验和 MAP2 表达分析评估了鱼藤酮的细胞毒性和神经元存活率。DCF-DA、罗丹明123(Rh-123)和DAPI分别测量细胞内活性氧(ROS)水平、线粒体膜电位(MMP)和凋亡核。此外,还评估了超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)和过氧化氢酶(CAT)的活性。对 STME 和 PAME 中的化合物进行了 LC-QTOF-MS 分析鉴定。研究表明,两种植物提取物(STME 和 PAME)在鱼藤酮诱导的神经毒性中都表现出抗氧化和神经保护能力,它们通过降低细胞内 ROS 水平来防止氧化应激,并逆转鱼藤酮引起的 GPx、SOD 和 CAT 活性。此外,这两种植物还能通过使 MMP 的稳态正常化和保护核 DNA 凝聚来防止细胞凋亡。LC-QTOF-MS 分析表明,STME 和 PAME 中分别含有尿苷和加巴喷丁等已知的神经保护化合物。这两种植物可能是具有神经保护能力的天然抗氧化剂和营养保健品的重要来源。可以进一步研究这两种植物,以配制治疗帕金森病等神经退行性疾病的草药保健品。
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来源期刊
Neurochemistry international
Neurochemistry international 医学-神经科学
CiteScore
8.40
自引率
2.40%
发文量
128
审稿时长
37 days
期刊介绍: Neurochemistry International is devoted to the rapid publication of outstanding original articles and timely reviews in neurochemistry. Manuscripts on a broad range of topics will be considered, including molecular and cellular neurochemistry, neuropharmacology and genetic aspects of CNS function, neuroimmunology, metabolism as well as the neurochemistry of neurological and psychiatric disorders of the CNS.
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