Preparation of ionic gel-modified stationary phase for RPLC/HILIC/IC separation and its application in per aqueous liquid chromatography

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS

Journal of Chromatography A Pub Date : 2024-08-31 DOI:10.1016/j.chroma.2024.465313

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Abstract

In this study, we synthesized and employed an ionic gel-functionalized silica stationary phase for high-performance liquid chromatography. The successful fabrication of the stationary phase was confirmed through attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), X-ray photoelectron spectroscopy (XPS), zeta-potential measurements, and elemental analysis (EA). Comparative performance evaluation against a commercial column demonstrated the prepared column's effectiveness in the mixed mode of reversed-phase liquid chromatography (RPLC), hydrophilic interaction liquid chromatography (HILIC), and ion chromatography (IC). Moreover, the stationary phase exhibited exceptional retention repeatability in per aqueous liquid chromatography, showcasing its potential as an environmentally friendly analytical method. Mechanistic investigations unveiled multiple solute-stationary phase interactions, including π-π interactions, hydrogen bonding, and ion exchange. Finally, we applied the developed stationary phase for the precise detection of preservatives in carbonated beverages and jelly, achieving high levels of accuracy and recovery rates.

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用于 RPLC/HILIC/IC 分离的离子凝胶改性固定相的制备及其在水相液相色谱中的应用。

在本研究中，我们合成并使用了一种离子凝胶功能化硅胶固定相，用于高效液相色谱。衰减全反射-傅立叶变换红外光谱（ATR-FTIR）、X 射线光电子能谱（XPS）、zeta 电位测量和元素分析（EA）证实了该固定相的成功制备。与商用色谱柱的性能对比评估表明，制备的色谱柱在反相液相色谱（RPLC）、亲水作用液相色谱（HILIC）和离子色谱（IC）混合模式下均有效。此外，该固定相在每水液相色谱法中表现出卓越的保留重复性，展示了其作为一种环境友好型分析方法的潜力。机理研究揭示了多种溶质-固定相相互作用，包括π-π相互作用、氢键和离子交换。最后，我们将所开发的固定相用于精确检测碳酸饮料和果冻中的防腐剂，实现了高准确度和高回收率。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.

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