Anti-tumor effects of nanosecond pulsed electric fields in a murine model of pancreatic cancer

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2024-08-31 DOI:10.1016/j.bioelechem.2024.108803

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引用次数: 0

Abstract

Nanosecond Pulsed Electric Fields (nsPEFs) treatment has demonstrated anti-tumor effects on various cancer cell lines. However, the use of this treatment in pancreatic cancer is limited. This study demonstrated that nsPEFs treatment effectively suppressed the proliferation and metastasis of pancreatic cancer cells, while also inducing DNA damage. Meanwhile, animal experiments have shown that nsPEFs effectively suppressed the growth of pancreatic cancer, even in cases where the tumor volume exceeded 500–600 mm³ at the initiation of treatment. Notably, a single treatment session was found to significantly inhibit tumor growth, while also showing no adverse effects on the main organs of the mice. RNA sequencing and bioinformatics revealed that seven key genes (CDK1, CENPA, UBE2C, CCNB2, PLK1, CCNA2, and CCNB14) were significantly correlated with the overall survival rate of patients with pancreatic cancer. Through the application of the competing endogenous RNA (ceRNA) hypothesis, two miRNAs (has-let-7b-5p and hsa-miR-193b-3p) and four lncRNAs (MIR4435-2HG, ZNF436-AS1, LINC01089, and MIR4435-2HG) were identified as significantly impacting the overall survival of pancreatic cancer patients. We have effectively developed an mRNA-miRNA-lncRNA network that has the potential to stimulate further investigation into the underlying mechanisms of nsPEFs on pancreatic cancer.

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纳秒脉冲电场在小鼠胰腺癌模型中的抗肿瘤作用

纳秒脉冲电场（nsPEFs）治疗已证明对多种癌细胞株有抗肿瘤作用。然而，这种疗法在胰腺癌中的应用还很有限。这项研究表明，nsPEFs 治疗能有效抑制胰腺癌细胞的增殖和转移，同时还能诱导 DNA 损伤。同时，动物实验表明，nsPEFs 能有效抑制胰腺癌的生长，即使治疗开始时肿瘤体积超过 500-600 立方毫米。值得注意的是，单次治疗就能显著抑制肿瘤生长，同时对小鼠的主要器官也没有不良影响。RNA测序和生物信息学发现，七个关键基因（CDK1、CENPA、UBE2C、CCNB2、PLK1、CCNA2和CCNB14）与胰腺癌患者的总生存率显著相关。通过应用竞争性内源性 RNA（ceRNA）假说，我们发现两个 miRNA（has-let-7b-5p 和 hsa-miR-193b-3p）和四个 lncRNA（MIR4435-2HG、ZNF436-AS1、LINC01089 和 MIR4435-2HG）对胰腺癌患者的总生存率有显著影响。我们有效地建立了一个 mRNA-miRNA-lncRNA 网络，该网络有可能激发人们进一步研究 nsPEFs 对胰腺癌的潜在影响机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.

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