Modulation of cutaneous vasodilation by reactive oxygen species during local and whole body heating in young and older adults.

IF 2.2 3区 医学 Q3 PHYSIOLOGY
Gregory W McGarr, Caroline Li-Maloney, Kelli E King, Kristina-Marie T Janetos, Naoto Fujii, Tatsuro Amano, Glen P Kenny
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引用次数: 0

Abstract

We evaluated reactive oxygen species (ROS) modulation of cutaneous vasodilation during local and whole body passive heating in young and older adults. Cutaneous vascular conductance normalized to maximum vasodilation (%CVCmax) was assessed in young and older adults (10/group) using laser-Doppler flowmetry at four dorsal forearm sites treated with 1) Ringer solution (control), 2) 100 µM apocynin (NADPH oxidase inhibitor), 3) 10 µM allopurinol (xanthine oxidase inhibitor), or 4) 10 µM tempol (superoxide dismutase mimetic), via intradermal microdialysis during local (protocol 1) and whole body heating (protocol 2). In protocol 1, forearm skin sites were set at 33°C during baseline and then progressively increased to 39°C and 42°C (30 min each). In protocol 2, participants were immersed in warm water (35°C, midsternum) with the experimental forearm above water level, and local skin sites were maintained at 34°C. Bath temperature was increased (∼40°C) to clamp core temperature at 38.5°C for 60 min. In protocol 1, there were significant treatment site by age interactions for the 39°C (P = 0.015) and 42°C (P = 0.004) plateaus; however no significant effects were observed after post hoc adjustment. In protocol 2, there was a significant treatment site by age interaction (P < 0.001), where %CVCmax in older adults was 11.0% [7.4, 14.6] higher for apocynin (P < 0.001), 8.9% [5.3, 12.5] higher for allopurinol (P < 0.001), and 4.8% [1.3, 8.4] higher for tempol (P = 0.016) sites relative to the control site. ROS derived from NADPH oxidase and xanthine oxidase attenuate cutaneous vasodilation in older adults during passive whole body heating, but not during local skin heating, with negligible effects on their young counterparts for either heating modality.NEW & NOTEWORTHY We found that local infusion of apocynin or allopurinol improved cutaneous vasodilator responses to passive whole body heating (but not local skin heating) in healthy older adults. These findings indicate that impaired microvascular responses to whole body heating with primary aging are linked to augmented production of reactive oxygen species (ROS) from NADPH oxidase and xanthine oxidase. This study sheds new light on the specific ROS pathways that modulate age-related changes in cutaneous microvascular responses to heating.

年轻人和老年人在局部和全身加热过程中活性氧对皮肤血管扩张的调节作用。
目的:评估活性氧(ROS)对年轻人和老年人在局部和全身被动加热过程中皮肤血管扩张的调节作用:评估活性氧(ROS)对年轻人和老年人在局部和全身被动加热过程中皮肤血管舒张的调节作用:方法:使用激光多普勒血流测量仪评估年轻人和老年人(每组 10 人)在 4 个前臂背侧部位的皮肤血管传导率归一化为最大血管舒张率(%CVCmax),处理方法包括:1)林格氏溶液(对照组);2)100 µM阿朴青宁(对照组);3)100 µM阿朴青宁(对照组);4)100 µM阿朴青宁(对照组)、2)100 µM阿朴霉素(NADPH 氧化酶抑制剂);3)10 µM别嘌醇(黄嘌呤氧化酶抑制剂);或 4)10 µM tempol(超氧化物歧化酶模拟物)。方案-1:前臂皮肤基线温度设定为 33°C,然后逐渐升至 39°C 和 42°C(各 30 分钟)。方案-2:将参与者浸入温水中(35°C,躯干中部),实验前臂高于水面,局部皮肤部位保持在 34°C。水浴温度升高(约 40°C),使核心温度保持在 38.5°C,持续 60 分钟:方案-1:39°C(P=0.015)和 42°C(P=0.004)高原存在显著的处理部位与年龄的交互作用。不过,经过事后调整后,没有观察到明显的影响。协议-2:治疗部位与年龄之间存在显著的交互作用(相对于对照部位,阿朴西宁(PP=0.016)治疗部位在老年人中的 Pmax 高 11.0% [7.4,14.6] :结论:在全身被动加热时,NADPH氧化酶和黄嘌呤氧化酶产生的ROS会减弱老年人的皮肤血管舒张,但在局部皮肤加热时不会,而在任何一种加热方式下对年轻人的影响都微乎其微。
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来源期刊
CiteScore
5.30
自引率
3.60%
发文量
145
审稿时长
2 months
期刊介绍: The American Journal of Physiology-Regulatory, Integrative and Comparative Physiology publishes original investigations that illuminate normal or abnormal regulation and integration of physiological mechanisms at all levels of biological organization, ranging from molecules to humans, including clinical investigations. Major areas of emphasis include regulation in genetically modified animals; model organisms; development and tissue plasticity; neurohumoral control of circulation and hypertension; local control of circulation; cardiac and renal integration; thirst and volume, electrolyte homeostasis; glucose homeostasis and energy balance; appetite and obesity; inflammation and cytokines; integrative physiology of pregnancy-parturition-lactation; and thermoregulation and adaptations to exercise and environmental stress.
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