Ze-Hao Hou , Yuan Gao , Jia-Cheng Zheng , Meng-Jie Zhao , Ying Liu , Xiao-Yu Cui , Zhi-Yong Li , Ji-Tong Wei , Tai-Fei Yu , Lei Zheng , Yuan-Chen Jiao , Shu-Hui Yang , Jia-Min Hao , Jun Chen , Yong-Bin Zhou , Ming Chen , Lijuan Qiu , You-Zhi Ma , Zhao-Shi Xu
{"title":"GmBSK1-GmGSK1-GmBES1.5 regulatory module controls heat tolerance in soybean","authors":"Ze-Hao Hou , Yuan Gao , Jia-Cheng Zheng , Meng-Jie Zhao , Ying Liu , Xiao-Yu Cui , Zhi-Yong Li , Ji-Tong Wei , Tai-Fei Yu , Lei Zheng , Yuan-Chen Jiao , Shu-Hui Yang , Jia-Min Hao , Jun Chen , Yong-Bin Zhou , Ming Chen , Lijuan Qiu , You-Zhi Ma , Zhao-Shi Xu","doi":"10.1016/j.jare.2024.09.004","DOIUrl":null,"url":null,"abstract":"<div><h3>Introduction</h3><div>Heat stress poses a severe threat to the growth and production of soybean (<em>Glycine</em> max). Brassinosteroids (BRs) actively participate in plant responses to abiotic stresses, however, the role of BR signaling pathway genes in response to heat stress in soybean remains poorly understood.</div></div><div><h3>Objectives</h3><div>In this study, we investigate the regulatory mechanisms of <em>GmBSK1</em> and <em>GmBES1.5</em> in response to heat stress and the physiological characteristics and yield performance under heat stress conditions.</div></div><div><h3>Methods</h3><div>Transgenic technology and CRISPR/Cas9 technology were used to generated <em>GmBSK1</em>-OE, <em>GmBES1.5</em>-OE and <em>gmbsk1</em> transgenic soybean plants, and transcriptome analysis, LUC activity assay and EMSA assay were carried out to elucidate the potential molecular mechanism underlying GmBSK1-GmBES1.5-mediated heat stress tolerance in soybean.</div></div><div><h3>Results</h3><div>CRISPR/Cas9-generated <em>gmbsk1</em> knockout mutants exhibited increased sensitivity to heat stress due to a reduction in their ability to scavenge reactive oxygen species (ROS). The expression of <em>GmBES1.5</em> was up-regulated in <em>GmBSK1</em>-OE plants under heat stress conditions, and it directly binds to the E-box motif present in the promoters of abiotic stress-related genes, thereby enhancing heat stress tolerance in soybean plants. Furthermore, we identified an interaction between GmGSK1 and GmBES1.5, while GmGSK1 inhibits the transcriptional activity of GmBES1.5. Interestingly, the interaction between GmBSK1 and GmGSK1 promotes the localization of GmGSK1 to the plasma membrane and releases the transcriptional activity of GmBES1.5.</div></div><div><h3>Conclusion</h3><div>Our findings suggest that both GmBSK1 and GmBES1.5 play crucial roles in conferring heat stress tolerance, highlighting a potential strategy for breeding heat-tolerant soybean crops involving the regulatory module consisting of GmBSK1-GmGSK1-GmBES1.5.</div></div>","PeriodicalId":14952,"journal":{"name":"Journal of Advanced Research","volume":"73 ","pages":"Pages 187-198"},"PeriodicalIF":11.4000,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Advanced Research","FirstCategoryId":"103","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2090123224003874","RegionNum":1,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MULTIDISCIPLINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Introduction
Heat stress poses a severe threat to the growth and production of soybean (Glycine max). Brassinosteroids (BRs) actively participate in plant responses to abiotic stresses, however, the role of BR signaling pathway genes in response to heat stress in soybean remains poorly understood.
Objectives
In this study, we investigate the regulatory mechanisms of GmBSK1 and GmBES1.5 in response to heat stress and the physiological characteristics and yield performance under heat stress conditions.
Methods
Transgenic technology and CRISPR/Cas9 technology were used to generated GmBSK1-OE, GmBES1.5-OE and gmbsk1 transgenic soybean plants, and transcriptome analysis, LUC activity assay and EMSA assay were carried out to elucidate the potential molecular mechanism underlying GmBSK1-GmBES1.5-mediated heat stress tolerance in soybean.
Results
CRISPR/Cas9-generated gmbsk1 knockout mutants exhibited increased sensitivity to heat stress due to a reduction in their ability to scavenge reactive oxygen species (ROS). The expression of GmBES1.5 was up-regulated in GmBSK1-OE plants under heat stress conditions, and it directly binds to the E-box motif present in the promoters of abiotic stress-related genes, thereby enhancing heat stress tolerance in soybean plants. Furthermore, we identified an interaction between GmGSK1 and GmBES1.5, while GmGSK1 inhibits the transcriptional activity of GmBES1.5. Interestingly, the interaction between GmBSK1 and GmGSK1 promotes the localization of GmGSK1 to the plasma membrane and releases the transcriptional activity of GmBES1.5.
Conclusion
Our findings suggest that both GmBSK1 and GmBES1.5 play crucial roles in conferring heat stress tolerance, highlighting a potential strategy for breeding heat-tolerant soybean crops involving the regulatory module consisting of GmBSK1-GmGSK1-GmBES1.5.
期刊介绍:
Journal of Advanced Research (J. Adv. Res.) is an applied/natural sciences, peer-reviewed journal that focuses on interdisciplinary research. The journal aims to contribute to applied research and knowledge worldwide through the publication of original and high-quality research articles in the fields of Medicine, Pharmaceutical Sciences, Dentistry, Physical Therapy, Veterinary Medicine, and Basic and Biological Sciences.
The following abstracting and indexing services cover the Journal of Advanced Research: PubMed/Medline, Essential Science Indicators, Web of Science, Scopus, PubMed Central, PubMed, Science Citation Index Expanded, Directory of Open Access Journals (DOAJ), and INSPEC.