Circulating microRNAs targeting coagulation and fibrinolysis in patients with severe COVID-19.

IF 2.6 4区医学 Q2 HEMATOLOGY

Thrombosis Journal Pub Date : 2024-09-05 DOI:10.1186/s12959-024-00649-w

Tuukka A Helin, Marja Lemponen, Katariina Immonen, Päivi Lakkisto, Lotta Joutsi-Korhonen

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引用次数: 0

Abstract

Background: Coronavirus-19 disease (COVID-19) frequently causes coagulation disturbances. Data remains limited on the effects of microRNAs (miRNAs) on coagulation during COVID-19 infection. We aimed to analyze the comprehensive miRNA profile as well as coagulation markers and blood count in hospitalized COVID-19 patients.

Methods: Citrated plasma samples from 40 patients (24 men and 16 women) hospitalized for COVID-19 were analyzed. Basic coagulation tests, von Willebrand factor (VWF), ADAMTS13, blood count, C-reactive protein, and 27 miRNAs known to associate with thrombosis or platelet activation were analyzed. MiRNAs were analyzed using quantitative reverse transcription polymerase chain reaction (RT qPCR), with 10 healthy controls serving as a comparator.

Results: Among the patients, 15/36 (41%) had platelet count of over 360 × 10⁹/L and 10/36 (28%) had low hemoglobin of < 100 g/L, while 26/37 (72%) had high VWF of over 200 IU/dL. Patients had higher levels of the miRNAs miR-27b-3p, miR-320a-3p, miR-320b-3p, and miR-424-5p, whereas levels of miR-103a-3p and miR-145-5p were lower than those in healthy controls. In total, 11 miRNAs were associated with platelet count. Let-7b-3p was associated with low hemoglobin levels of < 100 g/L. miR-24-3p, miR-27b-3p, miR-126-3p, miR-145-5p and miR-338-5p associated with high VWF.

Conclusion: COVID-19 patients differentially express miRNAs with target genes involved in fibrinolysis inhibition, coagulation activity, and increased inflammatory response. These findings support the notion that COVID-19 widely affects hemostasis, including platelets, coagulation and fibrinolysis.

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针对严重 COVID-19 患者凝血和纤维蛋白溶解的循环 microRNA。

背景：冠状病毒-19 疾病（COVID-19）经常导致凝血功能紊乱。有关微RNA（miRNA）在COVID-19感染期间对凝血功能影响的数据仍然有限。我们旨在分析住院COVID-19患者的miRNA综合谱以及凝血标志物和血细胞计数：方法：分析了 40 名 COVID-19 住院患者（24 名男性和 16 名女性）的枸橼酸血浆样本。分析了基本凝血试验、von Willebrand因子（VWF）、ADAMTS13、血细胞计数、C反应蛋白以及27种已知与血栓形成或血小板活化有关的miRNA。MiRNA 采用定量反转录聚合酶链反应（RT qPCR）进行分析，10 例健康对照组作为比较组：结果：在患者中，15/36（41%）人的血小板计数超过 360 × 109/L，10/36（28%）人的血红蛋白偏低：COVID-19 患者表达的 miRNA 与抑制纤溶、凝血活性和增加炎症反应的靶基因不同。这些发现支持了 COVID-19 广泛影响止血（包括血小板、凝血和纤溶）的观点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Thrombosis Journal Medicine-Hematology

CiteScore

3.80

自引率

3.20%

发文量

审稿时长

16 weeks

期刊介绍： Thrombosis Journal is an open-access journal that publishes original articles on aspects of clinical and basic research, new methodology, case reports and reviews in the areas of thrombosis. Topics of particular interest include the diagnosis of arterial and venous thrombosis, new antithrombotic treatments, new developments in the understanding, diagnosis and treatments of atherosclerotic vessel disease, relations between haemostasis and vascular disease, hypertension, diabetes, immunology and obesity.