Loop-mediated isothermal amplification (LAMP) for detection of atypical enterovirus D68 strain VR-1197

IF 2.2 4区医学 Q3 BIOCHEMICAL RESEARCH METHODS

Journal of virological methods Pub Date : 2024-09-03 DOI:10.1016/j.jviromet.2024.115030

Ann-Kristin Tveten, Hanne Lillerovde Ørstenvik, Ingvill Tolaas

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引用次数: 0

Abstract

A method that has rapidly evolved for detection of viral pathogens are loop-mediated isothermal amplification (LAMP) assays. The available LAMP assays usually target the most common viral strains, including enteroviruses, but for the atypical enterovirus D68 strain VR-1197 this method has not yet been developed. Enterovirus D68 are known for severe respiratory distress in children, and atypical strains are less likely to be detected by traditional methods. This study targets the atypical EVD68 strain VR-1197 and have developed a rapid detection method saving time when differentiating enterovirus strains. This study present method development and review the sensitivity and specificity compared to traditional RT-qPCR, and wet lab cross reactivity with other airway pathogens. The EVD68 VR-1197 assay can be a rapid POC (Point of care) test for atypical EVD68 VR-1197 and have the potential as reliable detection method with minimal technological requirements.

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用于检测非典型肠道病毒 D68 株 VR-1197 的环路介导等温扩增（LAMP）技术。

环路介导等温扩增（LAMP）检测法是一种快速发展的病毒病原体检测方法。现有的 LAMP 检测方法通常针对最常见的病毒株，包括肠道病毒，但针对非典型肠道病毒 D68 株 VR-1197 的这种方法尚未开发出来。众所周知，D68 型肠道病毒会导致儿童出现严重的呼吸困难，而非典型毒株不太可能通过传统方法检测出来。本研究针对非典型 EVD68 株 VR-1197，开发了一种快速检测方法，节省了区分肠道病毒株的时间。本研究介绍了方法的开发情况，并回顾了与传统 RT-qPCR 相比的灵敏度和特异性，以及与其他气道病原体的湿实验室交叉反应性。EVD68 VR-1197 检测试剂盒可作为非典型 EVD68 VR-1197 的快速 POC（护理点）检测试剂盒，具有作为可靠检测方法的潜力，且对技术要求极低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of virological methods 医学-病毒学

CiteScore

5.80

自引率

0.00%

发文量

209

审稿时长

41 days

期刊介绍： The Journal of Virological Methods focuses on original, high quality research papers that describe novel and comprehensively tested methods which enhance human, animal, plant, bacterial or environmental virology and prions research and discovery. The methods may include, but not limited to, the study of: Viral components and morphology- Virus isolation, propagation and development of viral vectors- Viral pathogenesis, oncogenesis, vaccines and antivirals- Virus replication, host-pathogen interactions and responses- Virus transmission, prevention, control and treatment- Viral metagenomics and virome- Virus ecology, adaption and evolution- Applied virology such as nanotechnology- Viral diagnosis with novelty and comprehensive evaluation. We seek articles, systematic reviews, meta-analyses and laboratory protocols that include comprehensive technical details with statistical confirmations that provide validations against current best practice, international standards or quality assurance programs and which advance knowledge in virology leading to improved medical, veterinary or agricultural practices and management.