Ayurveda Detoxification Process Reduces Plumbagin from the Roots of Plumbago zeylanica L. - A RP-UFLC Analysis.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-06 DOI:10.1093/chromsci/bmae048

Gireesh M Ankad, Harsha Hegde, Iranna B Kotturshetti

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Abstract

Ayurveda describes purification process of certain herbal drugs to reduce the toxicity and make them suitable for therapeutic purpose. The objective of the study was to evaluate the effect of detoxification process on plumbagin (PG) from the Plumbago zeylanica L. roots in marketed samples. It involved procurement of market samples from five states of India viz. Andhra Pradesh, Gujarat, Maharashtra, Madhya Pradesh and Punjab. The roots were purified in lime water (LW) as mentioned in Ayurveda. Reverse Phase Ultra-Flow Liquid Chromatography method was validated for identification of PG in unprocessed and processed roots and in the media (LW) used for purification after processing. The data was statistically analyzed by Analysis of Variance (ANOVA) and tested for significance by the Dunnett multiple comparison test. Results were expressed as mean ± SD mg/g dry weight of extract. The study indicated that the PG was reduced quantitatively after processing, while the amount of PG found in the LW was observed to be increased, indicating the leaching of PG during the purification process. In conclusion, the detoxification process eliminates PG from its roots and discloses the leaching effect in the media for the first time.

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阿育吠陀排毒过程减少了 Plumbago zeylanica L. 根中的 Plumbagin - RP-UFLC 分析。

阿育吠陀描述了某些草药的提纯过程，以降低毒性并使其适合治疗目的。这项研究的目的是评估解毒过程对市场样品中 Plumbago zeylanica L. 根中的 plumbagin (PG) 的影响。研究涉及从印度的五个邦（即安得拉邦、古吉拉特邦、马哈拉施特拉邦、中央邦和旁遮普邦）采购市场样品。按照阿育吠陀中的说法，根茎在石灰水（LW）中得到净化。对反相超流液相色谱法进行了验证，以鉴定未加工和加工根茎中的 PG，以及加工后用于净化的介质（石灰水）中的 PG。数据通过方差分析（ANOVA）进行统计分析，并通过邓尼特多重比较检验进行显著性检验。结果以平均值 ± SD 毫克/克提取物干重表示。研究表明，加工后 PG 的数量减少了，而观察到 LW 中 PG 的数量增加了，这表明在提纯过程中 PG 被浸出了。总之，解毒过程消除了根中的 PG，并首次揭示了介质中的浸出效应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.