Symbiotic and Nonsymbiotic Bacteria Associated With the Entomo-Pathogenic Nematode, Heterorhabditis spp (Rhabditida: Heterorhabditidae) From South India

IF 3.5 4区生物学 Q2 MICROBIOLOGY

Journal of Basic Microbiology Pub Date : 2024-09-06 DOI:10.1002/jobm.202400108

Athira G. Menon, Haseena Bhaskar, K. Surendra Gopal, Rajkumar M, Smitha M. Subramanian

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引用次数: 0

Abstract

Sixteen isolates of bacteria obtained from the entomopathogenic nematode (Heterorhabditis sp.) infected cadavers of Galleria mellonella larvae were identified following phenotypic characterization and molecular analysis of 16S rRNA. Two isolates were identified as the symbiotic bacterium, Photothabdus luminescens, while 14 other isolates were represented by nine species of nonsymbiotic bacteria viz., Stenotrophomonas maltophilia, Alcaligenes aquatilis, Brevundimonas diminuta, Brucella pseudointermedia, Ochrobactrum sp., Brucella pseudogrignonensis, Brucella anthropic, Pseudomonas azatoformans and Pseudomonas lactis. The phylogenetic analysis confirmed the evolutionary relationship between P. luminescens and Pseudomonas spp. The study also found a close relationship among the nonsymbiotic bacteria such as A. aquatilis, B. diminuta, Ochrobactrum sp., and Brucella spp. P. luminescens has been documented for its insecticidal effects against a wide range of insects. The two local isolates obtained in this study may be explored for their biocontrol potential against major pests of the region. Further, the association of nonsymbiotic bacteria with the EPN may be investigated.

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与南印度昆虫病原线虫 Heterorhabditis spp（Rhabditida: Heterorhabditidae）相关的共生和非共生细菌。

通过表型鉴定和 16S rRNA 分子分析，鉴定了从受昆虫病原线虫（Heterorhabditis sp.）感染的 Galleria mellonella 幼虫尸体中分离出的 16 个细菌。其中 2 个分离物被鉴定为共生细菌 Photothabdus luminescens，另外 14 个分离物由 9 种非共生细菌代表，即嗜麦芽气单胞菌、水生嗜水气单胞菌、Brevundimonas diminuta、Brucella pseudointermedia、Ochrobactrum sp.、Brucella pseudogrignonensis、Brucella anthropic、Pseudomonas azatoformans 和 Pseudomonas lactis。系统进化分析证实了 P. luminescens 与假单胞菌属之间的进化关系。研究还发现，非共生细菌（如 A. aquatilis、B. diminuta、Ochrobactrum sp.本研究中获得的两个本地分离物可用于探索其对该地区主要害虫的生物防治潜力。此外，还可以研究非共生细菌与 EPN 的关联。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Basic Microbiology 生物-微生物学

CiteScore

6.10

自引率

0.00%

发文量

134

审稿时长

1.8 months

期刊介绍： The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).