Symbiotic and Nonsymbiotic Bacteria Associated With the Entomo-Pathogenic Nematode, Heterorhabditis spp (Rhabditida: Heterorhabditidae) From South India
Athira G. Menon, Haseena Bhaskar, K. Surendra Gopal, Rajkumar M, Smitha M. Subramanian
{"title":"Symbiotic and Nonsymbiotic Bacteria Associated With the Entomo-Pathogenic Nematode, Heterorhabditis spp (Rhabditida: Heterorhabditidae) From South India","authors":"Athira G. Menon, Haseena Bhaskar, K. Surendra Gopal, Rajkumar M, Smitha M. Subramanian","doi":"10.1002/jobm.202400108","DOIUrl":null,"url":null,"abstract":"<div>\n \n <p>Sixteen isolates of bacteria obtained from the entomopathogenic nematode (<i>Heterorhabditis</i> sp.) infected cadavers of <i>Galleria mellonella</i> larvae were identified following phenotypic characterization and molecular analysis of <i>16S rRNA</i>. Two isolates were identified as the symbiotic bacterium, <i>Photothabdus luminescens</i>, while 14 other isolates were represented by nine species of nonsymbiotic bacteria viz., <i>Stenotrophomonas maltophilia</i>, <i>Alcaligenes aquatilis, Brevundimonas diminuta</i>, <i>Brucella pseudointermedia</i>, <i>Ochrobactrum</i> sp., <i>Brucella pseudogrignonensis</i>, <i>Brucella anthropic</i>, <i>Pseudomonas azatoformans</i> and <i>Pseudomonas lactis</i>. The phylogenetic analysis confirmed the evolutionary relationship between <i>P. luminescens</i> and <i>Pseudomonas</i> spp. The study also found a close relationship among the nonsymbiotic bacteria such as <i>A. aquatilis</i>, <i>B. diminuta, Ochrobactrum</i> sp., and <i>Brucell</i>a spp. <i>P. luminescens</i> has been documented for its insecticidal effects against a wide range of insects. The two local isolates obtained in this study may be explored for their biocontrol potential against major pests of the region. Further, the association of nonsymbiotic bacteria with the EPN may be investigated.</p>\n </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Basic Microbiology","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jobm.202400108","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Sixteen isolates of bacteria obtained from the entomopathogenic nematode (Heterorhabditis sp.) infected cadavers of Galleria mellonella larvae were identified following phenotypic characterization and molecular analysis of 16S rRNA. Two isolates were identified as the symbiotic bacterium, Photothabdus luminescens, while 14 other isolates were represented by nine species of nonsymbiotic bacteria viz., Stenotrophomonas maltophilia, Alcaligenes aquatilis, Brevundimonas diminuta, Brucella pseudointermedia, Ochrobactrum sp., Brucella pseudogrignonensis, Brucella anthropic, Pseudomonas azatoformans and Pseudomonas lactis. The phylogenetic analysis confirmed the evolutionary relationship between P. luminescens and Pseudomonas spp. The study also found a close relationship among the nonsymbiotic bacteria such as A. aquatilis, B. diminuta, Ochrobactrum sp., and Brucella spp. P. luminescens has been documented for its insecticidal effects against a wide range of insects. The two local isolates obtained in this study may be explored for their biocontrol potential against major pests of the region. Further, the association of nonsymbiotic bacteria with the EPN may be investigated.
期刊介绍:
The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions.
Papers published deal with:
microbial interactions (pathogenic, mutualistic, environmental),
ecology,
physiology,
genetics and cell biology/development,
new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications)
novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).