Brazil nut (Bertholletia excelsa) and metformin abrogate cardiac complication in fructose/STZ-induced type 2 diabetic rats by attenuating oxidative stress and modulating the MAPK-mTOR/NFkB/IL-10 signaling pathways.

IF 3.5 4区 医学 Q2 FOOD SCIENCE & TECHNOLOGY
Food & Nutrition Research Pub Date : 2024-08-20 eCollection Date: 2024-01-01 DOI:10.29219/fnr.v68.10749
Zhenzuo Li, Baolan Wang, Dongfang Bai, Li Zhang
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引用次数: 0

Abstract

Background: The global prevalence of diabetic heart complication has been on the increase, and some of the drugs that are currently used to treat diabetes mellitus (DM) have not been able to mitigate this complication.

Objective: This study determines the effect of Brazil nut (Bertholletia excelsa) and metformin on diabetic cardiomyopathy (DCM) in fructose/streptozotocin (STZ)-induced type 2 diabetic rats and also characterizes using Gas Chromatography Mass Spectrophotometry and Fourier Transform Infrared the bioactive compounds in 50% aqueous ethanol extract of Brazil nut.

Design: After inducing type 2 DM, 30 male albino Wistar rats were separated into five groups that comprised of six rats per group, and they were treated as follows: groups 1 (Control) and 2 (Diabetic control) rats received rat pellets and distilled water; group 3 (Diabetic + Brazil nut) received rat pellets and Brazil nut extract (100 mg/kg, orally) dissolved in distilled water, group 4 (Diabetic + metformin) received metformin (100 mg/kg, orally) dissolved in distilled water, while group 5 (Diabetic + Brazil nut + metformin) received oral administrations of Brazil nut (100 mg/kg) and metformin (100 mg/kg) dissolved in distilled water. This study lasted for 6 weeks. The dose of Brazil nut used was selected from our pilot study on the minimum therapeutic dose of different concentrations of Brazil nut extract.

Results: STZ administration induced insulin resistance, hyperglycemia, loss of weight, dyslipidemia, oxidative stress, inflammation, apoptosis, alteration of mammalian target of rapamycin, mitogen-activated protein kinase, heart function markers (creatine kinase MB, lactate dehydrogenase, and aspartate amino transaminase), and heart histology of the diabetic control, which was ameliorated after treatment with Brazil nut and metformin, but their combined treatment was better than the single treatments.

Conclusion: This study shows that Brazil nut contains several bioactive compounds that support its biological properties as well as its candidature as a complementary therapy to metformin in mitigating cardiac complications arising from DM in rats.

巴西坚果(Bertholletia excelsa)和二甲双胍通过减轻氧化应激和调节 MAPK-mTOR/NFkB/IL-10 信号通路,减轻果糖/STZ 诱导的 2 型糖尿病大鼠的心脏并发症。
背景:全球糖尿病心脏并发症的发病率呈上升趋势,而目前用于治疗糖尿病(DM)的一些药物并不能缓解这一并发症:本研究确定了巴西坚果(Bertholletia excelsa)和二甲双胍对果糖/链脲佐菌素(STZ)诱导的 2 型糖尿病大鼠的糖尿病心肌病(DCM)的影响,并使用气相色谱质谱仪和傅立叶变换红外光谱分析了巴西坚果 50% 水乙醇提取物中的生物活性化合物:诱导 2 型糖尿病后,将 30 只雄性白化 Wistar 大鼠分为 5 组,每组 6 只,处理方法如下:第 1 组(对照组)和第 2 组(糖尿病对照组)大鼠接受大鼠颗粒和蒸馏水;第 3 组(糖尿病 + 巴西坚果)接受溶于蒸馏水的大鼠颗粒和巴西坚果提取物(100 毫克/千克,口服);第 4 组(糖尿病 + 二甲双胍)接受溶于蒸馏水的二甲双胍(100 毫克/千克,口服);第 5 组(糖尿病 + 巴西坚果 + 二甲双胍)接受溶于蒸馏水的巴西坚果(100 毫克/千克)和二甲双胍(100 毫克/千克)口服。这项研究持续了 6 周。巴西坚果的剂量是根据我们对不同浓度巴西坚果提取物的最小治疗剂量进行的试验研究选定的:结果:STZ 会诱发糖尿病对照组的胰岛素抵抗、高血糖、体重下降、血脂异常、氧化应激、炎症、细胞凋亡、雷帕霉素哺乳动物靶标的改变、丝裂原活化蛋白激酶、心脏功能指标(肌酸激酶 MB、乳酸脱氢酶和天冬氨酸氨基转氨酶)和心脏组织学,而巴西坚果和二甲双胍治疗后这些症状会得到改善,但两者联合治疗的效果优于单一治疗。结论这项研究表明,巴西坚果含有多种生物活性化合物,这些化合物支持巴西坚果的生物特性,并支持巴西坚果作为二甲双胍的辅助疗法,以减轻大鼠因糖尿病引起的心脏并发症。
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来源期刊
Food & Nutrition Research
Food & Nutrition Research FOOD SCIENCE & TECHNOLOGY-NUTRITION & DIETETICS
CiteScore
5.20
自引率
9.10%
发文量
47
审稿时长
14 weeks
期刊介绍: Food & Nutrition Research is a peer-reviewed journal that presents the latest scientific research in various fields focusing on human nutrition. The journal publishes both quantitative and qualitative research papers. Through an Open Access publishing model, Food & Nutrition Research opens an important forum for researchers from academic and private arenas to exchange the latest results from research on human nutrition in a broad sense, both original papers and reviews, including: * Associations and effects of foods and nutrients on health * Dietary patterns and health * Molecular nutrition * Health claims on foods * Nutrition and cognitive functions * Nutritional effects of food composition and processing * Nutrition in developing countries * Animal and in vitro models with clear relevance for human nutrition * Nutrition and the Environment * Food and Nutrition Education * Nutrition and Economics Research papers on food chemistry (focus on chemical composition and analysis of foods) are generally not considered eligible, unless the results have a clear impact on human nutrition. The journal focuses on the different aspects of nutrition for people involved in nutrition research such as Dentists, Dieticians, Medical doctors, Nutritionists, Teachers, Journalists and Manufacturers in the food and pharmaceutical industries.
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