Temperature dependence of the protective effect of pressurized dissolution of xenon gas during cold storage of cells.

IF 1 4区 医学 Q4 ENGINEERING, BIOMEDICAL
Kenshi Mimura, Rina Sakai, Kazuhiro Yoshida, Masanobu Ujihira
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引用次数: 0

Abstract

Background: The preservation period afforded by cold storage of cells is short. However, the use of rare gases for cold storage as a means of extending the period of preservation would be highly beneficial.

Objective: To examine the effect of temperature on the protective effect of cold storage of cells using pressurized dissolution of xenon gas, with particular focus on the inhibition of substance transport by viscosity.

Methods: Human dermal fibroblast monolayers incubated in a culture dish for 48 h were used as a test sample, with culture medium used as a preservation solution. Samples were placed into a pressure-resistant vessel, which was pressurized with xenon gas at 0 or 0.5 MPa, and cells were stored at 0 to 5 °C for 18 h. Cell activity was evaluated by tetrazolium salt assay. The viscosity of the medium under pressurization at each storage temperature was estimated.

Results: The maximum protective effect against cell damage of cold storage with pressurized dissolution of xenon gas was observed at 4 °C. An increase in estimated viscosity by pressurization was correlated with increased cell activity at 4 °C.

Conclusion: Analysis of the temperature dependence of the protective effect against cell damage of cold storage with pressurized dissolution of xenon gas revealed that the most effective temperature is 4 °C. The data also suggest that increased viscosity due to pressurization plays a role in the protective effect.

细胞冷藏期间加压溶解氙气的保护作用与温度有关。
背景:细胞冷藏的保存期很短。然而,使用稀有气体进行冷藏作为延长保存期的一种手段将大有裨益:研究温度对利用加压溶解氙气冷藏细胞的保护作用的影响,尤其关注粘度对物质运输的抑制作用。方法:以在培养皿中培养 48 小时的人真皮成纤维细胞单层为测试样本,培养基为保存液。将样品放入耐压容器中,用 0 或 0.5 兆帕的氙气对容器加压,细胞在 0 至 5 °C 下保存 18 小时。对每个储存温度下加压培养基的粘度进行了估算:结果:氙气加压溶解冷藏对细胞损伤的最大保护作用出现在 4 °C。在 4 °C时,加压估计粘度的增加与细胞活性的增加相关:分析加压溶解氙气冷藏对细胞损伤的保护作用与温度的关系发现,最有效的温度是 4 °C。数据还表明,加压导致的粘度增加在保护作用中起了一定作用。
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来源期刊
Bio-medical materials and engineering
Bio-medical materials and engineering 工程技术-材料科学:生物材料
CiteScore
1.80
自引率
0.00%
发文量
73
审稿时长
6 months
期刊介绍: The aim of Bio-Medical Materials and Engineering is to promote the welfare of humans and to help them keep healthy. This international journal is an interdisciplinary journal that publishes original research papers, review articles and brief notes on materials and engineering for biological and medical systems. Articles in this peer-reviewed journal cover a wide range of topics, including, but not limited to: Engineering as applied to improving diagnosis, therapy, and prevention of disease and injury, and better substitutes for damaged or disabled human organs; Studies of biomaterial interactions with the human body, bio-compatibility, interfacial and interaction problems; Biomechanical behavior under biological and/or medical conditions; Mechanical and biological properties of membrane biomaterials; Cellular and tissue engineering, physiological, biophysical, biochemical bioengineering aspects; Implant failure fields and degradation of implants. Biomimetics engineering and materials including system analysis as supporter for aged people and as rehabilitation; Bioengineering and materials technology as applied to the decontamination against environmental problems; Biosensors, bioreactors, bioprocess instrumentation and control system; Application to food engineering; Standardization problems on biomaterials and related products; Assessment of reliability and safety of biomedical materials and man-machine systems; and Product liability of biomaterials and related products.
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