Temperature control technology for PCR

IF 1.6 4区工程技术 Q3 ENGINEERING, ELECTRICAL & ELECTRONIC

International Journal of Numerical Modelling-Electronic Networks Devices and Fields Pub Date : 2024-09-03 DOI:10.1002/jnm.3280

Tongxin Zhang, Gaozhe Cai, Zhuo Zhang, Qian Li, Chuanjin Cui

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引用次数: 0

Abstract

In order to solve the obvious nonlinear problem of temperature and complex structure of PCR instrument during nucleic acid amplification. In this paper, a new nucleic acid amplification device and temperature control algorithm were proposed. In the device, in order to improve the rise and fall rate and make the whole reaction device smaller and simpler, this paper uses a microfluidic chip for nucleic acid reaction. At the same time, in the warming and cooling module, the temperature is controlled by the semiconductor chilling plate, the air-cooled cooling device and the heat sink structure, which greatly improves the speed of nucleic acid amplification. In the algorithm, a hybrid algorithm is designed, using Particle Swarm Optimization (PSO) to optimize PID algorithm parameters, and then based on fuzzy theory, according to the temperature control requirements of nucleic acid amplification, fuzzy rules are analyzed and fuzzy reasoning is carried out, and then combined with PID to achieve rapid response and overshooting control of temperature control. Finally, the measurement noise is filtered by Kalman filter. Finally, COMSOL and MATLAB software are used to simulate and compare, and it is proved that the device has a certain heat dissipation effect in the process of nucleic acid amplification. This algorithm can improve the accuracy and robustness of the control system, improve the response speed, reduce the overshoot, shorten the adjustment time, and restrain the interference.

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用于 PCR 的温度控制技术

为了解决核酸扩增过程中温度与 PCR 仪器结构复杂这一明显的非线性问题。本文提出了一种新型核酸扩增装置和温度控制算法。在装置中，为了提高升降温速率，使整个反应装置更小更简单，本文采用了微流控芯片进行核酸反应。同时，在升温降温模块中，通过半导体制冷板、风冷散热装置和散热片结构控制温度，大大提高了核酸扩增的速度。在算法上，设计了一种混合算法，利用粒子群优化（PSO）优化 PID 算法参数，然后基于模糊理论，根据核酸扩增的温度控制要求，分析模糊规则，进行模糊推理，再与 PID 结合，实现温度控制的快速响应和超调控制。最后，利用卡尔曼滤波器对测量噪声进行过滤。最后，利用 COMSOL 和 MATLAB 软件进行仿真对比，证明该装置在核酸扩增过程中具有一定的散热效果。该算法可以提高控制系统的精度和鲁棒性，提高响应速度，减少过冲，缩短调节时间，抑制干扰。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Numerical Modelling-Electronic Networks Devices and Fields 工程技术-工程：电子与电气

CiteScore

4.60

自引率

6.20%

发文量

101

审稿时长

>12 weeks

期刊介绍： Prediction through modelling forms the basis of engineering design. The computational power at the fingertips of the professional engineer is increasing enormously and techniques for computer simulation are changing rapidly. Engineers need models which relate to their design area and which are adaptable to new design concepts. They also need efficient and friendly ways of presenting, viewing and transmitting the data associated with their models. The International Journal of Numerical Modelling: Electronic Networks, Devices and Fields provides a communication vehicle for numerical modelling methods and data preparation methods associated with electrical and electronic circuits and fields. It concentrates on numerical modelling rather than abstract numerical mathematics. Contributions on numerical modelling will cover the entire subject of electrical and electronic engineering. They will range from electrical distribution networks to integrated circuits on VLSI design, and from static electric and magnetic fields through microwaves to optical design. They will also include the use of electrical networks as a modelling medium.