Developmental regulators in promoting genetic transformation efficiency in maize and other plants

IF 5.4 Q1 PLANT SCIENCES
Yilin Jiang , Xun Wei , Meirui Zhu , Xiaoyan Zhang , Qingping Jiang , ZiXiao Wang , Yanyong Cao , Xueli An , Xiangyuan Wan
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Abstract

Given global agricultural challenges such as population growth, climate change, and limitations on resources and the environment, as well as increasing diversity in breeding goals, relying on traditional breeding methods is inadequate to provide food security requirements and promote sustainable development. Genetic transformation technology has become an effective tool for performing functional genomics research and molecular breeding. In this study, we conducted an in-depth analysis of 1669 literary works to investigate the potential of developmental regulators (DRs) in enhancing the efficiency of plant genetic transformation, with a concentration on their use in maize. Through multi-omics data analysis, we identified 12 homologous DRs from various species that are potentially applicable to maize. We identified a total of 41 possible disease resistances (DRs) for maize genetic transformation. Further experimental verification of ZmWIND1, a novel regulator belonging to the ERF/AP2 transcription factor (TF) family, showed that it significantly improved the efficiency of plant regeneration and transformation efficiency in maize. Specifically, compared to the control group, the callus induction rates for the pG3GB411-ZmWIND1 vector increased to 60.22 % and 47.85 % in Xiang249 and Zheng58, respectively. Transformation efficiency increased to 37.5 % in Xiang249 and 16.56 % in Zheng58, both significantly surpassing the control group. These findings have the potential to broaden the range of transformable maize varieties and lines, as well as introduce new genetic transformation methods in agricultural biotechnology, underscoring the immense potential to enhance genetic transformation efficiency through systematic exploration and application of DRs in maize.

促进玉米和其他植物遗传转化效率的发育调节因子
鉴于人口增长、气候变化、资源和环境限制等全球农业挑战,以及育种目标的日益多样化,依靠传统的育种方法已不足以满足粮食安全需求和促进可持续发展。遗传转化技术已成为进行功能基因组学研究和分子育种的有效工具。在本研究中,我们对 1669 篇文学作品进行了深入分析,以研究发育调节剂(DRs)在提高植物遗传转化效率方面的潜力,并重点关注其在玉米中的应用。通过多组学数据分析,我们从不同物种中发现了 12 种可能适用于玉米的同源 DRs。我们共发现了 41 种可能用于玉米基因转化的抗病性(DRs)。ZmWIND1是一种属于ERF/AP2转录因子(TF)家族的新型调控因子,进一步的实验验证表明,它能显著提高玉米的植株再生效率和转化效率。具体而言,与对照组相比,pG3GB411-ZmWIND1载体在湘249和郑58中的胼胝体诱导率分别提高到60.22%和47.85%。向249和郑58的转化效率分别提高到37.5%和16.56%,均显著超过对照组。这些发现有望扩大可转化玉米品种和品系的范围,并为农业生物技术引入新的遗传转化方法,凸显了通过系统探索和应用 DRs 提高玉米遗传转化效率的巨大潜力。
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来源期刊
Current Plant Biology
Current Plant Biology Agricultural and Biological Sciences-Plant Science
CiteScore
10.90
自引率
1.90%
发文量
32
审稿时长
50 days
期刊介绍: Current Plant Biology aims to acknowledge and encourage interdisciplinary research in fundamental plant sciences with scope to address crop improvement, biodiversity, nutrition and human health. It publishes review articles, original research papers, method papers and short articles in plant research fields, such as systems biology, cell biology, genetics, epigenetics, mathematical modeling, signal transduction, plant-microbe interactions, synthetic biology, developmental biology, biochemistry, molecular biology, physiology, biotechnologies, bioinformatics and plant genomic resources.
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