In vivo overexpression of the avian interleukin-17 in a necrotic enteritis disease model modulates the expression of antimicrobial peptides in the small intestine of broilers

IF 3.7 3区 医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
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引用次数: 0

Abstract

In humans and mice, the induction of interleukin (IL)-17 expression enhances epithelial barrier integrity through the secretion of antimicrobial peptides (AMP), thereby improving antibacterial defense. However, it is unclear whether IL-17 has similar antibacterial effects in chickens by modulating the expression of AMPs, such as avian beta-defensins (also known as gallinacins) and cathelicidins. This study evaluated the in vivo effects of inoculating 20-day-old broiler chickens with two doses of a plasmid encoding chicken IL-17 (pCDNA3.1/rchIL-17-V5-HIS TOPO plasmid [pCDNA3.1-IL-17]; 5 or 10 μg/bird). On day 23 of age, all broilers, except those in the negative control group, were orally challenged with a virulent Clostridium perfringens strain for three days. To investigate IL-17-mediated effects against C. perfringens infection, the expression of avian beta-defensin 1 (avBD1), avBD2, avBD4, avBD6, cathelicidins, and inducible nitric oxide synthase (iNOS) genes were quantified, and gross necrotic enteritis (NE) lesion scores were assessed in the small intestine. The results showed that broilers receiving the higher dose of pCDNA3.1-IL-17 (10 μg) had significantly lower NE lesion scores compared to those receiving the lower dose (5 μg), the vector control, and the positive control groups. Furthermore, the expression of all avian beta-defensins and cathelicidin genes was detectable across all groups, regardless of treatment and time points. IL-17 treatment led to significantly higher expression of avBD1, avBD2, avBD4, avBD6, cathelicidin, and iNOS in the duodenum, jejunum, and ileum compared to control chickens. In C. perfringens-infected chickens, the expression of avBD1, avBD2, avBD4, cathelicidin, and iNOS in the ileum was significantly higher than in control chickens. Pre-treatment with the higher dose of pCDNA3.1-IL-17 (10 μg) in infected chickens was associated with reduced NE lesion severity and increased expression of avBD1, avBD2, cathelicidin, and iNOS in the ileum, but not avBD4 and avBD6. These findings provide new insights into the potential effect of IL-17 and reduction in NE lesion severity by modulating AMP expression which may be involved in mediating protective immunity against intestinal infection with C. perfringens.

在坏死性肠炎疾病模型中体内过表达禽白细胞介素-17可调节肉鸡小肠中抗菌肽的表达
在人类和小鼠体内,诱导白细胞介素(IL)-17 的表达可通过分泌抗菌肽(AMP)增强上皮屏障的完整性,从而提高抗菌防御能力。然而,目前还不清楚 IL-17 是否也能通过调节 AMPs(如禽类 beta-防御素(又称五倍子肽)和白头翁肽)的表达对鸡产生类似的抗菌作用。本研究评估了用两种剂量的编码鸡 IL-17 的质粒(pCDNA3.1/rchIL-17-V5-HIS TOPO 质粒 [pCDNA3.1-IL-17];5 或 10 μg/只)接种 20 日龄肉鸡的体内效应。第 23 日龄时,除阴性对照组外,所有肉鸡均口服产气荚膜梭菌毒株 3 天。为了研究IL-17介导的抗产气荚膜梭菌感染的作用,对禽β防御素1(avBD1)、avBD2、avBD4、avBD6、柔毛素和诱导型一氧化氮合酶(iNOS)基因的表达进行了量化,并评估了小肠坏死性肠炎(NE)的病变评分。结果表明,与接受低剂量(5 μg)pCDNA3.1-IL-17的肉鸡、载体对照组和阳性对照组相比,接受高剂量(10 μg)pCDNA3.1-IL-17的肉鸡的NE病变评分明显较低。此外,无论治疗方法和时间点如何,所有组别都能检测到所有禽类β-防御素和chelicidin基因的表达。与对照组相比,IL-17 处理导致十二指肠、空肠和回肠中 avBD1、avBD2、avBD4、avBD6、chelicidin 和 iNOS 的表达量明显增加。在受产气荚膜杆菌感染的鸡中,回肠中 avBD1、avBD2、avBD4、chelicidin 和 iNOS 的表达量明显高于对照组。用较高剂量的 pCDNA3.1-IL-17(10 μg)预处理感染鸡,可降低 NE 病变的严重程度,增加回肠中 avBD1、avBD2、cathelicidin 和 iNOS 的表达,但不增加 avBD4 和 avBD6 的表达。这些发现提供了新的视角,让我们了解到 IL-17 通过调节 AMP 的表达来降低 NE 病变严重程度的潜在作用,而 AMP 的表达可能参与介导保护性免疫,防止肠道感染产气荚膜杆菌。
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来源期刊
Cytokine
Cytokine 医学-免疫学
CiteScore
7.60
自引率
2.60%
发文量
262
审稿时长
48 days
期刊介绍: The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.
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