Focusing on tumor and it’s microenvironmental immune members for head and neck cancer patients

IF 2.9 4区医学 Q2 PATHOLOGY

Pathology, research and practice Pub Date : 2024-08-30 DOI:10.1016/j.prp.2024.155575

{"title":"Focusing on tumor and it’s microenvironmental immune members for head and neck cancer patients","authors":"","doi":"10.1016/j.prp.2024.155575","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>Immune-related gene expression levels in the tumor microenvironment (TM) of head and neck squamous cell carcinoma (HNSCC) patients was compared.</p></div><div><h3>Materials and methods</h3><p>The <em>CD163</em>, <em>CD274</em>, <em>CD86</em>, <em>FUT4</em>, <em>FOXP3</em>, and <em>ITGAX</em> levels of HNSCC patients in their tumor tissues (n =76) and surrounding tissues adjacent to the tumor (n =76) were determined using quantitative real-time PCR (qRT-PCR). Changes in these genes were also evaluated by associating with demographical data of the patients.</p></div><div><h3>Results</h3><p><em>CD163</em>, <em>CD274</em>, <em>FUT4,</em> and <em>FOXP3</em> gene expression levels were significantly higher in tumor tissue than in surrounding tissue. <em>FUT4</em> fold change was statistically higher in patients with lymph node involvement. <em>CD86</em> expression was statistically lower in smokers of 50 boxes per year or more. <em>CD163</em>, <em>CD274</em>, and <em>FUT4</em> expressions were increased in response to the presence of extranodal extension (ENE).</p></div><div><h3>Conclusions</h3><p>These preliminary results demonstrate the alterations in expression levels of immunologic markers are associated with the clinical presentations of HNSCC.</p></div><div><h3>Availability of data and materials</h3><p>The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.</p></div>","PeriodicalId":19916,"journal":{"name":"Pathology, research and practice","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pathology, research and practice","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0344033824004862","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PATHOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objective

Immune-related gene expression levels in the tumor microenvironment (TM) of head and neck squamous cell carcinoma (HNSCC) patients was compared.

Materials and methods

The CD163, CD274, CD86, FUT4, FOXP3, and ITGAX levels of HNSCC patients in their tumor tissues (n =76) and surrounding tissues adjacent to the tumor (n =76) were determined using quantitative real-time PCR (qRT-PCR). Changes in these genes were also evaluated by associating with demographical data of the patients.

Results

CD163, CD274, FUT4, and FOXP3 gene expression levels were significantly higher in tumor tissue than in surrounding tissue. FUT4 fold change was statistically higher in patients with lymph node involvement. CD86 expression was statistically lower in smokers of 50 boxes per year or more. CD163, CD274, and FUT4 expressions were increased in response to the presence of extranodal extension (ENE).

Conclusions

These preliminary results demonstrate the alterations in expression levels of immunologic markers are associated with the clinical presentations of HNSCC.

Availability of data and materials

The datasets used and/or analysed during the current study available from the corresponding author on reasonable request.

查看原文本刊更多论文

关注头颈部癌症患者的肿瘤及其微环境免疫成员

目的比较头颈部鳞状细胞癌（HNSCC）患者肿瘤微环境（TM）中免疫相关基因的表达水平。材料和方法采用定量实时 PCR（qRT-PCR）技术测定 HNSCC 患者肿瘤组织（n =76）和肿瘤邻近周围组织（n =76）中的 CD163、CD274、CD86、FUT4、FOXP3 和 ITGAX 水平。结果CD163、CD274、FUT4和FOXP3基因在肿瘤组织中的表达水平明显高于周围组织。据统计，淋巴结受累的患者中 FUT4 的折叠变化更高。据统计，每年吸烟 50 盒或以上者的 CD86 表达较低。CD163、CD274和FUT4的表达随结节外扩展（ENE）的出现而增加。结论这些初步结果表明，免疫标志物表达水平的改变与HNSCC的临床表现有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pathology, research and practice 医学-病理学

CiteScore

5.00

自引率

3.60%

发文量

405

审稿时长

24 days

期刊介绍： Pathology, Research and Practice provides accessible coverage of the most recent developments across the entire field of pathology: Reviews focus on recent progress in pathology, while Comments look at interesting current problems and at hypotheses for future developments in pathology. Original Papers present novel findings on all aspects of general, anatomic and molecular pathology. Rapid Communications inform readers on preliminary findings that may be relevant for further studies and need to be communicated quickly. Teaching Cases look at new aspects or special diagnostic problems of diseases and at case reports relevant for the pathologist''s practice.

文献相关原料

公司名称	产品信息	采购帮参考价格