Two +ssRNA mycoviruses cohabiting the fungal cultivar of leafcutter ants.

IF 4 3区医学 Q2 VIROLOGY

Virology Journal Pub Date : 2024-09-04 DOI:10.1186/s12985-024-02465-0

Asta Rødsgaard-Jørgensen, Caio Ambrosio Leal-Dutra, Sabrina Ferreira de Santana, Asger Roland Jensen, Rafael Elias Marques, Eric Roberto Guimarães Rocha Aguiar, Jonathan Zvi Shik

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Abstract

Leafcutter ants are dominant herbivores in the Neotropics and rely on a fungus (Leucoagaricus gongylophorus) to transform freshly gathered leaves into a source of nourishment rather than consuming the vegetation directly. Here we report two virus-like particles that were isolated from L. gongylophorus and observed using transmission electron microscopy. RNA sequencing identified two +ssRNA mycovirus strains, Leucoagaricus gongylophorus tymo-like virus 1 (LgTlV1) and Leucoagaricus gongylophorus magoulivirus 1 (LgMV1). Genome annotation of LgTlV1 (7401 nt) showed conserved domains for methyltransferase, endopeptidase, viral RNA helicase, and RNA-dependent RNA polymerase (RdRp). The smaller genome of LgMV1 (2636 nt) contains one open reading frame encoding an RdRp. While we hypothesize these mycoviruses function as symbionts in leafcutter farming systems, further study will be needed to test whether they are mutualists, commensals, or parasites.

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两种+ssRNA霉形体病毒与切叶蚁的真菌栽培种同居。

切叶蚁是新热带地区的主要食草动物，它们依靠一种真菌（Leucoagaricus gongylophorus）将新采集的树叶转化为营养来源，而不是直接食用植被。在此，我们报告了从鼯鼠体内分离出的两种病毒样颗粒，并使用透射电子显微镜对其进行了观察。RNA 测序确定了两种+ssRNA霉菌病毒株，即褐飞虱tymo-like病毒1（LgTlV1）和褐飞虱magoulivirus 1（LgMV1）。LgTlV1 的基因组注释（7 401 nt）显示了甲基转移酶、内肽酶、病毒 RNA 螺旋酶和 RNA 依赖性 RNA 聚合酶（RdRp）的保守结构域。较小的 LgMV1 基因组（2636 nt）包含一个编码 RdRp 的开放阅读框。虽然我们推测这些霉菌病毒在剪叶虫养殖系统中具有共生体的功能，但还需要进一步的研究来检验它们是互生体、共生体还是寄生虫。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Virology Journal 医学-病毒学

CiteScore

7.40

自引率

2.10%

发文量

186

审稿时长

1 months

期刊介绍： Virology Journal is an open access, peer reviewed journal that considers articles on all aspects of virology, including research on the viruses of animals, plants and microbes. The journal welcomes basic research as well as pre-clinical and clinical studies of novel diagnostic tools, vaccines and anti-viral therapies. The Editorial policy of Virology Journal is to publish all research which is assessed by peer reviewers to be a coherent and sound addition to the scientific literature, and puts less emphasis on interest levels or perceived impact.