Diagnostic value for methylation in cervical cancer based on a small-molecule fluorescent probe targeting DNMT1.

IF 2 4区医学 Q3 DERMATOLOGY

Skin Research and Technology Pub Date : 2024-09-01 DOI:10.1111/srt.70042

Baohua Yang, Chao Xu, Hang Li, Xiuxiang Zhu, Ziyin Xia, Ling Xu, Qian Zhang

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Abstract

Background: Value analysis of a small-molecule fluorescent probe for methylation detection in different cervical lesions.

Materials and methods: (1) The grayscale values of distinct lesion tissues were remarkably distinct among the four groups (p < 0.05). The comparison of the grayscale value between the two groups showed that the CA group noticeably exceeded the LSIL and cervicitis groups, and the HSIL group was apparently higher than the LSIL and cervicitis groups (p < 0.05); (2) The mean grayscale values of the enrolled subjects were calculated with 55.21 as the midline, with >55.21 as positive and ≤55.21 as negative.

Results: The results showed that the positive rate of the cervicitis group was 0.00%, the LSIL group 67.74%, the HSIL group 83.33%, and the CA group 100.00%. The results among the four groups were notably distinct (p < 0.05); (3) The comparison among DAPI, probe, bright, and merged images of cervicitis, LSIL, HSIL, and CA indicated that different cervical lesions were with quite various stains.

Conclusion: The grayscale value, positive rate, and stained picture of distinct cervical lesions were remarkably different. The small-molecule fluorescent probe has a good value in differentiating cervical lesions and can be considered for popularization and application.

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基于靶向 DNMT1 的小分子荧光探针的宫颈癌甲基化诊断价值。

背景：小分子荧光探针在不同宫颈病变中甲基化检测的价值分析。材料与方法：（1）四组病变组织灰度值差异明显（p 55.21为阳性，≤55.21为阴性），结果显示，宫颈炎组阳性率为0.00%，LSIL组为67.74%，HSIL组为83.33%，CA组为100.00%：结果显示，宫颈炎组阳性率为 0.00%，LSIL 组为 67.74%，HSIL 组为 83.33%，CA 组为 100.00%。四组之间的结果差异明显（P不同宫颈病变的灰度值、阳性率和染色图明显不同。小分子荧光探针在鉴别宫颈病变方面有较好的价值，可考虑推广应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Skin Research and Technology 医学-皮肤病学

CiteScore

3.30

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Skin Research and Technology is a clinically-oriented journal on biophysical methods and imaging techniques and how they are used in dermatology, cosmetology and plastic surgery for noninvasive quantification of skin structure and functions. Papers are invited on the development and validation of methods and their application in the characterization of diseased, abnormal and normal skin. Topics include blood flow, colorimetry, thermography, evaporimetry, epidermal humidity, desquamation, profilometry, skin mechanics, epiluminiscence microscopy, high-frequency ultrasonography, confocal microscopy, digital imaging, image analysis and computerized evaluation and magnetic resonance. Noninvasive biochemical methods (such as lipids, keratin and tissue water) and the instrumental evaluation of cytological and histological samples are also covered. The journal has a wide scope and aims to link scientists, clinical researchers and technicians through original articles, communications, editorials and commentaries, letters, reviews, announcements and news. Contributions should be clear, experimentally sound and novel.