Poly(G)7 box: a functional element of mammalian 18S rRNA involved in translation.

IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
RNA Biology Pub Date : 2024-01-01 Epub Date: 2024-09-05 DOI:10.1080/15476286.2024.2399310
Dahao Wei, Zhangyu Mai, Xinan Li, Tianli Yu, Jiangchao Li
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引用次数: 0

Abstract

In eukaryotes, the ribosomal small subunit (40S) is composed of 18S rRNA and 33 ribosomal proteins. 18S rRNA has a special secondary structure and is an indispensable part of the translation process. Herein, a special sequence located in mammalian 18S rRNA named Poly(G)7box, which is composed of seven guanines, was found. Poly(G)7 can form a special and stable secondary structure by binding to the translation elongation factor subunit eEF1D and the ribosomal protein RPL32. Poly(G)7box was transfected into cells, and the translation efficiency of cells was inhibited. We believe that Poly(G)7box is an important translation-related functional element located on mammalian 18S rRNA, meanwhile the Poly(G)7 located on mRNA 5' and 3' box does not affect mRNA translation.

Poly(G)7 box:哺乳动物 18S rRNA 中参与翻译的一个功能元件。
在真核生物中,核糖体小亚基(40S)由 18S rRNA 和 33 种核糖体蛋白组成。18S rRNA 具有特殊的二级结构,是翻译过程中不可或缺的一部分。在这里,我们发现了哺乳动物 18S rRNA 中的一个特殊序列,名为 Poly(G)7box ,由 7 个鸟嘌呤组成。Poly(G)7 能与翻译延伸因子亚基 eEF1D 和核糖体蛋白 RPL32 结合,形成特殊而稳定的二级结构。将 Poly(G)7box 转染细胞后,细胞的翻译效率受到抑制。我们认为Poly(G)7box是位于哺乳动物18S rRNA上的一个重要的翻译相关功能元件,而位于mRNA 5'和3'框上的Poly(G)7并不影响mRNA的翻译。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
RNA Biology
RNA Biology 生物-生化与分子生物学
CiteScore
8.60
自引率
0.00%
发文量
82
审稿时长
1 months
期刊介绍: RNA has played a central role in all cellular processes since the beginning of life: decoding the genome, regulating gene expression, mediating molecular interactions, catalyzing chemical reactions. RNA Biology, as a leading journal in the field, provides a platform for presenting and discussing cutting-edge RNA research. RNA Biology brings together a multidisciplinary community of scientists working in the areas of: Transcription and splicing Post-transcriptional regulation of gene expression Non-coding RNAs RNA localization Translation and catalysis by RNA Structural biology Bioinformatics RNA in disease and therapy
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