Identification and functional analysis of C-type lectin from mosquito Aedes albopictus in response to dengue virus infection.

IF 3 2区医学 Q1 PARASITOLOGY

Parasites & Vectors Pub Date : 2024-09-04 DOI:10.1186/s13071-024-06453-9

Sheng Gao, Haodong Xu, Hongbo Li, Xiao Feng, Jitao Zhou, Renxian Guo, Zihan Liang, Jinying Ding, Xin Li, Yijia Huang, Wenquan Liu, Shaohui Liang

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引用次数: 0

Abstract

Background: C-type lectins (CTLs) are a large family of proteins with sugar-binding activity. CTLs contain an evolutionarily conserved C-type lectin domain (CTLD) that binds microbial carbohydrates in a calcium-dependent manner, thereby playing a key role in both microbial pathogenesis and innate immune responses. Aedes albopictus is an important vector for transmitting dengue virus (DENV) worldwide. Currently, the molecular characteristics and functions of CTLs in Ae. albopictus are largely unknown.

Methods: Transcripts encoding CTL proteins in the Ae. albopictus genome assembly were analyzed via sequence blast. Phylogenetic analysis and molecular characterization were performed to identify the functional domains of the CTLs. Quantitative analysis was performed to determine the gene expression features of CTLs during mosquito development and in different tissues of female adults after blood feeding. In addition, the functional role of CTLs in response to DENV infection was investigated in Ae. albopictus mosquito cells.

Results: We identified 39 transcripts encoding CTL proteins in the Ae. albopictus transcriptome. Aedes albopictus CTLs are classified into three groups based on the number of CTLDs and the domain architecture. These included 29 CTL-Ss (single-CTLDs), 1 immulectins (dual-CTLD) and 9 CTL-Xs (CTLDs with other domains). Phylogenetic analysis and structural modeling indicated that CTLs in Ae. albopictus are highly conserved with the homologous CTLs in Aedes aegypti. The expression profile assay revealed differential expression patterns of CTLs in both developmental stages and in adult female tissues. Knockdown and overexpression of three CTLs (CTL-S12, S17 and S19) confirmed that they can promote dengue virus infection in Ae. albopictus cells.

Conclusions: The CTL genes in Ae. albopictus mosquito and other mosquito species are evolutionarily conserved and exhibit different developmental and tissue expression features. The functional assay indicated that three CTLs in Ae. albopictus mosquitoes are involved in promoting dengue virus infection. Our study revealed that CTLs play important roles in both the physiological processes and viral infection in mosquito vectors.

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白纹伊蚊对登革热病毒感染反应的 C 型凝集素的鉴定和功能分析。

背景：C型凝集素（CTL）是一大类具有糖结合活性的蛋白质。CTLs含有一个进化保守的C型凝集素结构域（CTLD），能以钙依赖方式结合微生物碳水化合物，从而在微生物致病和先天免疫反应中发挥关键作用。白纹伊蚊是全球登革热病毒（DENV）的重要传播媒介。目前，白纹伊蚊 CTL 的分子特征和功能在很大程度上还不清楚：方法：通过序列爆炸分析了白纹伊蚊基因组中编码 CTL 蛋白的转录本。进行了系统发育分析和分子鉴定，以确定 CTL 的功能域。定量分析确定了 CTLs 在蚊子发育过程中和雌成虫吸血后在不同组织中的基因表达特征。此外，还研究了白纹伊蚊细胞中 CTLs 在应对 DENV 感染时的功能作用：结果：我们在白纹伊蚊转录组中发现了 39 个编码 CTL 蛋白的转录本。根据 CTLD 的数量和结构域将白纹伊蚊 CTL 分成三组。其中包括 29 个 CTL-S（单 CTLD）、1 个 immulectins（双 CTLD）和 9 个 CTL-X（具有其他结构域的 CTLD）。系统发育分析和结构建模表明，白纹伊蚊的 CTL 与埃及伊蚊的同源 CTL 高度保守。表达谱分析揭示了 CTLs 在发育阶段和成年雌虫组织中的不同表达模式。三个 CTLs（CTL-S12、S17 和 S19）的敲除和过表达证实它们能促进登革病毒在白纹伊蚊细胞中的感染：白纹伊蚊和其他蚊子物种的 CTL 基因在进化上是保守的，并表现出不同的发育和组织表达特征。功能检测表明，白纹伊蚊的三种 CTLs 参与促进登革病毒感染。我们的研究揭示了 CTLs 在蚊媒的生理过程和病毒感染中都发挥着重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Parasites & Vectors 医学-寄生虫学

CiteScore

6.30

自引率

9.40%

发文量

433

审稿时长

1.4 months

期刊介绍： Parasites & Vectors is an open access, peer-reviewed online journal dealing with the biology of parasites, parasitic diseases, intermediate hosts, vectors and vector-borne pathogens. Manuscripts published in this journal will be available to all worldwide, with no barriers to access, immediately following acceptance. However, authors retain the copyright of their material and may use it, or distribute it, as they wish. Manuscripts on all aspects of the basic and applied biology of parasites, intermediate hosts, vectors and vector-borne pathogens will be considered. In addition to the traditional and well-established areas of science in these fields, we also aim to provide a vehicle for publication of the rapidly developing resources and technology in parasite, intermediate host and vector genomics and their impacts on biological research. We are able to publish large datasets and extensive results, frequently associated with genomic and post-genomic technologies, which are not readily accommodated in traditional journals. Manuscripts addressing broader issues, for example economics, social sciences and global climate change in relation to parasites, vectors and disease control, are also welcomed.