Characteristics of Recombinant Chlamydomonas reinhardtii Expressing Putative Germin-Like Protein from Neopyropia yezoensis.

IF 2.5 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Journal of microbiology and biotechnology Pub Date : 2024-10-28 Epub Date: 2024-08-14 DOI:10.4014/jmb.2407.07059
Jiae Kim, Jong-Il Choi
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引用次数: 0

Abstract

Since microalgae face various environmental stresses for the high production of biofuels, multiple studies have been performed to determine if microalgae are resistant to these various stresses. In this study, the viability of cells under various abiotic stresses was investigated by introducing a putative germin-like protein (GLP) from Neopyropia yezoensis, which was known to be related in the resistance to abiotic stresses. The expression of GLP in Chlamydomonas reinhardtii allowed cells to grow better in various abiotic stress environments. In nitrogen starvation conditions, recombinant cells accumulated the lipid droplet 1.46-fold more than wild-type cells and responded more rapidly to form palmelloid forms. Under high-temperature, hydrogen peroxide conditions and saline stress, the survival rate was increased 3.5 times, 2.19 times, and 3.19 times in recombinant C. reinhardtii with GLP, respectively. The expression level of genes related to pathways in response to various stresses increased 2-fold more under those conditions. This result will be useful for the development of microalgae that can grow better and produce more biofuels under different stress conditions.

表达来自新叶藻的拟胚芽鞘蛋白的重组衣藻的特征
由于微藻类在大量生产生物燃料的过程中面临着各种环境压力,因此已经开展了多项研究,以确定微藻类是否能抵抗这些不同的压力。在本研究中,通过引入一种来自 Neopyropia yezoensis 的假定胚芽鞘样蛋白(GLP),研究了细胞在各种非生物胁迫下的活力。在莱茵衣藻(Chlamydomonas reinhardtii)中表达 GLP 能使细胞在各种非生物胁迫环境中更好地生长。在氮饥饿条件下,重组细胞积累的脂滴是野生型细胞的1.46倍,并能更快地形成棕榈状。在高温、过氧化氢和盐水胁迫条件下,GLP 重组 C. reinhardtii 的存活率分别提高了 3.5 倍、2.19 倍和 3.19 倍。在这些条件下,与应对各种胁迫的途径相关的基因表达水平增加了 2 倍。这一结果将有助于开发在不同胁迫条件下能生长得更好、生产更多生物燃料的微藻类。
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来源期刊
Journal of microbiology and biotechnology
Journal of microbiology and biotechnology BIOTECHNOLOGY & APPLIED MICROBIOLOGY-MICROBIOLOGY
CiteScore
5.50
自引率
3.60%
发文量
151
审稿时长
2 months
期刊介绍: The Journal of Microbiology and Biotechnology (JMB) is a monthly international journal devoted to the advancement and dissemination of scientific knowledge pertaining to microbiology, biotechnology, and related academic disciplines. It covers various scientific and technological aspects of Molecular and Cellular Microbiology, Environmental Microbiology and Biotechnology, Food Biotechnology, and Biotechnology and Bioengineering (subcategories are listed below). Launched in March 1991, the JMB is published by the Korean Society for Microbiology and Biotechnology (KMB) and distributed worldwide.
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