45S5 Bioactive Glass-Ointment Positively Effects on Wound Healing in Rats by Regulating TNFα, Il-10, VEGF, and TGFβ

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2024-09-05 DOI:10.1002/jcla.25094

Ömer Kırgız, Muhammed Enes Altuğ, Hüseyin Özkan, Mehmet Cengiz Han, Gökhan Akçakavak, Ali Can Özarslan, Sevil Yücel

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Abstract

Aim

This study aimed to investigate the effects of 45S5 bioactive glass-ointment (BG) on cutaneous wound healing in rats at the molecular, biochemical, and histopathological levels.

Materials and Methods

Thirty-two rats were divided into four groups (n = 8): Control, Sham, BG, and DEX (Dexpanthenol). While no wound treatment was applied to the CONTROL, a wound model was created in the Sham, and no treatment was applied. A wound model was created for other groups, and BG and DEX were applied locally for 21 days. During the 21-day experiment period, feed and water consumption and weight changes were observed. Wound areas were calculated on days 0, 3, 7, 4, and 21. Following treatment, the rats were euthanized and tissues from the wound area and blood samples were collected. While the expression levels of tumor necrosis factor-alpha (TNFα), Interleukin 6 (IL6), Interleukin 10 (IL10), transforming growth factor-beta (TGFβ), and vascular endothelial growth factor (VEGF) genes were determined by qPCR, the levels of TNFα, IL6, and IL10 proteins were measured by ELISA.

Results

It was observed that the BG group showed anti-inflammatory activity by suppressing TNFα levels and stimulating IL-10. In addition, it was determined that BG increased fibroblast activity and vascularization.

Conclusion

Current findings showed that topical application of BG has anti-inflammatory effects, while also accelerating healing by increasing vascularity and making positive contributions to tissue healing.

Abstract Image

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45S5 生物活性玻璃软膏通过调节 TNFα、Il-10、VEGF 和 TGFβ 对大鼠伤口愈合产生积极影响

目的：本研究旨在从分子、生物化学和组织病理学水平研究 45S5 生物活性玻璃膏（BG）对大鼠皮肤伤口愈合的影响：将 32 只大鼠分为四组（n = 8）：材料：32 只大鼠被分为四组（n = 8）：对照组、虚弱组、BG 组和 DEX（右泛醇）组。对照组不进行任何伤口处理，而 Sham 组则创建了一个伤口模型，也不进行任何处理。为其他组创建伤口模型，并在局部施用 BG 和 DEX，持续 21 天。在 21 天的实验期间，观察饲料和水的消耗量以及体重变化。在第 0、3、7、4 和 21 天计算伤口面积。治疗后，对大鼠实施安乐死，并收集伤口部位的组织和血液样本。采用 qPCR 法测定肿瘤坏死因子-α（TNFα）、白细胞介素 6（IL6）、白细胞介素 10（IL10）、转化生长因子-β（TGFβ）和血管内皮生长因子（VEGF）基因的表达水平，采用 ELISA 法测定 TNFα、IL6 和 IL10 蛋白的水平：结果：观察发现，BG 组通过抑制 TNFα 水平和刺激 IL-10 显示出抗炎活性。此外，BG 还能增强成纤维细胞的活性和血管生成：目前的研究结果表明，局部应用生物碱具有抗炎作用，同时还能通过增加血管来加速愈合，并对组织愈合做出积极贡献。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.