Evaluation of amlodipine against strains of Candida spp. in planktonic cells, developing biofilms and mature biofilms.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2024-09-05 DOI:10.1080/17460913.2024.2390286

Helaine Almeida Queiroz, Lisandra Juvêncio da Silva, Fátima Daiana Dias Barroso, Lívia Gurgel do Amaral Valente Sá, João Batista de Andrade Neto, Érica Rayanne Motta da Costa, Leilson Carvalho de Oliveira, Amanda Dias Barbosa, Vitória Pessoa de Farias Cabral, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira, Bruno Coêlho Cavalcanti, Islay Lima Magalhães, Manoel Odorico de Moraes, Hélio Vitoriano Nobre Júnior, Cecília Rocha da Silva

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Abstract

Aim: To evaluate the antifungal activity of amlodipine against strains of Candida spp. and to its possible mechanism of action.Methods: Broth microdilution tests were used to determine the minimum inhibitory concentration, while the synergistic activity was evaluated by calculating the fractional inhibitory concentration index. The action of amlodipine against biofilms was determined using the MTT assay and its possible mechanism of action was investigated through flow cytometry tests.Results: Amlodipine showed MICs ranging from 62.5 to 250 μg/ml, in addition to action against pre-formed and forming biofilms, with reductions between 50 and 90%. Amlodipine increases the externalization of phosphatidylserine and reduces the cell viability of fungal cells, suggesting apoptosis.Conclusion: Amlodipine had good antifungal activity against planktonic cells and biofilms of Candida spp., by leading the cells to apoptosis.

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评估氨氯地平对浮游细胞、发育中生物膜和成熟生物膜中念珠菌菌株的作用。

目的：评估氨氯地平对念珠菌菌株的抗真菌活性及其可能的作用机制：方法：采用肉汤微稀释试验确定最低抑菌浓度，并通过计算部分抑菌浓度指数评估协同活性。使用 MTT 试验确定氨氯地平对生物膜的作用，并通过流式细胞仪测试研究其可能的作用机制：结果：氨氯地平的 MIC 值在 62.5 到 250 μg/ml 之间，此外还对已形成和正在形成的生物膜有作用，可减少 50% 到 90% 的生物膜。氨氯地平增加了磷脂酰丝氨酸的外化，降低了真菌细胞的活力，提示了细胞凋亡：结论：氨氯地平通过引导细胞凋亡，对白色念珠菌属的浮游细胞和生物膜具有良好的抗真菌活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.