Phenotypic and Genotypic of Antimicrobial Resistance Profile of Staphylococcus Spp. Isolated from Raw Milk and Artisanal Cheese.

IF 1.9 2区农林科学 Q3 FOOD SCIENCE & TECHNOLOGY

Foodborne pathogens and disease Pub Date : 2024-09-05 DOI:10.1089/fpd.2024.0037

Fernanda Danielle Melo, Heloíse Peterle, Ricardo Antonio Pilegi Sfaciotte, Mateus Felipe Schneider, Leandro Parussolo, Sheila Rezler Wosiacki, Ubirajara Maciel da Costa, Sandra Maria Ferraz

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Abstract

Staphylococcus aureus and a few species of coagulase negative are frequently associated with food poisoning. Raw milk and dairy products are among the foods usually associated with outbreaks due to staphylococcal intoxication. This study aimed to determine phenotypic and genotypic antimicrobial resistance profiles to beta-lactam drugs in Staphylococcus coagulase positive (CoPS) and negative (CoNS) isolates. A total of 58 CoPS and 45 CoNS isolates recovered from raw milk and artisanal cheese from Santa Catarina were analyzed. All isolates (n = 103) were subjected to antimicrobial susceptibility testing. High levels of resistance to penicillin (41% of CoPS and 31% of CoNS), amoxicillin (40% CoPS), ampicillin (36% CoPS), and sulfamethoxazole-trimethoprim (35% CoNS) were observed. Twenty six percent of the isolates (18 CoPS and 9 CoNS) exhibited multiresistance profile; which means, they were resistant to at least three different classes of the antimicrobial drugs. Detection of resistance genes (mecA, mecC, and blaZ) was performed using multiplex polymerase chain reaction. Twelve isolates (9 CoPS and 3 CoNS) were positive for mecA, whereas 10 strains (4 CoPS and 6 CoNS) were positive for blaZ. The detection of resistant and multidrug resistant isolates emphasizes the necessity to develop strategies to better comply with good manufacturing practices and health care guidelines.

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从生乳和手工奶酪中分离的葡萄球菌的抗菌谱表型和基因型从生乳和手工奶酪中分离的葡萄球菌的抗菌谱。

金黄色葡萄球菌和少数几种凝固酶阴性的葡萄球菌经常与食物中毒有关。生奶和奶制品是通常与葡萄球菌中毒爆发有关的食品之一。本研究旨在确定凝固酶阳性葡萄球菌（CoPS）和阴性葡萄球菌（CoNS）分离物对β-内酰胺类药物的表型和基因型抗菌谱。对从圣卡塔琳娜州的生牛奶和手工奶酪中分离出的 58 株 CoPS 和 45 株 CoNS 进行了分析。对所有分离物（n = 103）进行了抗菌药敏感性测试。观察到对青霉素（41% 的 CoPS 和 31% 的 CoNS）、阿莫西林（40% 的 CoPS）、氨苄西林（36% 的 CoPS）和磺胺甲噁唑-三甲氧苄啶（35% 的 CoNS）具有高度耐药性。26%的分离物（18 CoPS 和 9 CoNS）表现出多重耐药性特征；这意味着它们至少对三种不同类别的抗菌药物具有耐药性。耐药基因（mecA、mecC 和 blaZ）的检测采用多重聚合酶链反应法进行。12 个分离株（9 个 CoPS 和 3 个 CoNS）的 mecA 呈阳性，10 个分离株（4 个 CoPS 和 6 个 CoNS）的 blaZ 呈阳性。耐药性和耐多药分离株的检测结果表明，有必要制定战略，以更好地遵守良好生产规范和医疗保健指南。

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来源期刊

Foodborne pathogens and disease 医学-食品科技

CiteScore

5.30

自引率

3.60%

发文量

审稿时长

1 months

期刊介绍： Foodborne Pathogens and Disease is one of the most inclusive scientific publications on the many disciplines that contribute to food safety. Spanning an array of issues from "farm-to-fork," the Journal bridges the gap between science and policy to reduce the burden of foodborne illness worldwide. Foodborne Pathogens and Disease coverage includes: Agroterrorism Safety of organically grown and genetically modified foods Emerging pathogens Emergence of drug resistance Methods and technology for rapid and accurate detection Strategies to destroy or control foodborne pathogens Novel strategies for the prevention and control of plant and animal diseases that impact food safety Biosecurity issues and the implications of new regulatory guidelines Impact of changing lifestyles and consumer demands on food safety.