Microbiome and Femoral Cartilage Thickness in Knee Osteoarthritis: Is There a Link?

IF 2.7 4区医学 Q1 ORTHOPEDICS

CARTILAGE Pub Date : 2024-09-05 DOI:10.1177/19476035241276852

Noha Abdelhalim Elsawy, Aya Hanafy Ibrahiem, Gihan Abdellatif Younis, Marwa Ahmed Meheissen, Yousra Hisham Abdel-Fattah

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引用次数: 0

Abstract

Objective: To assess the relation between microbiome and lipopolysaccharide (LPS), in the blood and synovial fluid (SF) with femoral cartilage thickness (FCT) measured by ultrasound (US) in knee osteoarthritis (KOA) patients.

Methods: This cross-sectional study included 40 primary KOA patients recruited between September 2022 and June 2023. Age, gender, and body mass index (BMI) were recorded. Patients underwent full clinical examination, standing plain x-ray of the knee joint and knee US examination to measure medial, intercondylar, and lateral FCT. Microbiomes (specific bacterial phyla) were detected by real-time polymerase chain reaction and LPS levels were measured by enzyme-linked immunosorbent assay kit in the patients' serum and SF.

Results: The patient's age ranged from 43 to 72 years. Most patients were females (72.5%), with a mean BMI of 35.8 ± 6.21 kg/m². The mean medial, intercondylar, and lateral FCT were less than cut-off values. All 40 (100%) patients showed positive bacterial deoxyribonucleic acid (16S ribosomal RNA) in both blood and SF samples. Firmicutes was the most abundant in patients' blood (48.49%) and SF (63.59%). The mean serum LPS level was significantly higher compared to mean SF LPS (t =4.702, P < 0.001). There was a statistically significant negative correlation between lateral FCT and Firmicutes relative abundance in both patients' blood and SF.

Conclusion: Microbiome and LPS are present in the blood and SF of primary KOA patients. Microbiome (Firmicutes) was associated with decreased lateral FCT. This might provide a potential link between both systemic and local microbiomes and cartilage affection in KOA patients.

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膝骨关节炎患者的微生物组和股骨软骨厚度：两者之间有联系吗？

目的评估膝骨关节炎（KOA）患者血液和滑液（SF）中微生物组和脂多糖（LPS）与超声波（US）测量的股骨头软骨厚度（FCT）之间的关系：这项横断面研究纳入了2022年9月至2023年6月间招募的40名原发性KOA患者。研究记录了患者的年龄、性别和体重指数（BMI）。患者接受了全面的临床检查、膝关节立位X光平片检查和膝关节US检查，以测量内侧、髁间和外侧的FCT。通过实时聚合酶链反应检测微生物组（特定细菌门），并通过酶联免疫吸附测定试剂盒检测患者血清和 SF 中的 LPS 水平：患者年龄从 43 岁到 72 岁不等。大多数患者为女性（72.5%），平均体重指数（BMI）为 35.8 ± 6.21 kg/m2。内侧、髁间和外侧的平均 FCT 均小于临界值。所有 40 名患者（100%）的血液和 SF 样本中的细菌脱氧核糖核酸（16S 核糖体 RNA）均呈阳性。患者血液（48.49%）和自体培养样本（63.59%）中最多的是真菌。平均血清 LPS 水平明显高于平均 SF LPS 水平（t =4.702，P <0.001）。侧向 FCT 与患者血液和 SF 中的真菌相对丰度之间存在统计学意义上的负相关：结论：原发性 KOA 患者的血液和 SF 中存在微生物组和 LPS。结论：原发性 KOA 患者的血液和 SF 中存在微生物组和 LPS，微生物组（真菌）与侧向 FCT 的减少有关。这可能是全身和局部微生物群与 KOA 患者软骨损伤之间的潜在联系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

CARTILAGE ORTHOPEDICS-

CiteScore

6.90

自引率

7.10%

发文量

期刊介绍： CARTILAGE publishes articles related to the musculoskeletal system with particular attention to cartilage repair, development, function, degeneration, transplantation, and rehabilitation. The journal is a forum for the exchange of ideas for the many types of researchers and clinicians involved in cartilage biology and repair. A primary objective of CARTILAGE is to foster the cross-fertilization of the findings between clinical and basic sciences throughout the various disciplines involved in cartilage repair. The journal publishes full length original manuscripts on all types of cartilage including articular, nasal, auricular, tracheal/bronchial, and intervertebral disc fibrocartilage. Manuscripts on clinical and laboratory research are welcome. Review articles, editorials, and letters are also encouraged. The ICRS envisages CARTILAGE as a forum for the exchange of knowledge among clinicians, scientists, patients, and researchers. The International Cartilage Repair Society (ICRS) is dedicated to promotion, encouragement, and distribution of fundamental and applied research of cartilage in order to permit a better knowledge of function and dysfunction of articular cartilage and its repair.