Investigation of physiological roles of UDP-glycosyltransferase UGT76F2 in auxin homeostasis through the TAA-YUCCA auxin biosynthesis pathway.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-04 DOI:10.1093/bbb/zbae124

Mio Harada, Tomoaki Kubotsu, Takemoto Agui, Xinhua Dai, Yunde Zhao, Hiroyuki Kasahara, Ken-Ichiro Hayashi

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引用次数: 0

Abstract

Cellular auxin (indole-3-acetic acid, IAA) levels are coordinately regulated by IAA biosynthesis and inactivation. IAA is synthesized through sequential reactions by two enzymes, TAA1 and YUCCA, in a linear indole-3-pyruvic acid (IPA) pathway. TAA1 converts tryptophan to IPA, and YUCCA catalyzes the oxidative decarboxylation of IPA into IAA. Arabidopsis UDP-glycosyltransferase UGT76F2 (At3g55710) was previously reported to catalyze the glycosylation of IPA and consequently modulate IAA levels. We carefully analyzed the physiological roles of UGT76F2 and its close homolog UGT76F1 (At3g55700) in IAA homeostasis. We generated two independent ugt76f1 ugt76f2 double null Arabidopsis mutants (ugt76f1f2) with a 2.7 kb deletion, along with two independent ugt76f2 single null mutants by CRISPR/Cas9 gene editing technology. Surprisingly, these null mutants exhibited indistinguishable phenotypes from the wild-type seedlings under our laboratory conditions. Our results indicate that UGT76F1 and UGT76F2 do not play important roles in regulating IAA biosynthesis via IPA glycosylation.

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研究 UDP-糖基转移酶 UGT76F2 通过 TAA-YUCCA auxin 生物合成途径在 auxin 平衡中的生理作用。

细胞辅助素（吲哚-3-乙酸，IAA）水平受 IAA 生物合成和失活的协调调节。在吲哚-3-丙酮酸（IPA）线性途径中，IAA通过 TAA1 和 YUCCA 两种酶的连续反应合成。TAA1 将色氨酸转化为 IPA，YUCCA 催化 IPA 氧化脱羧转化为 IAA。据报道，拟南芥 UDP-糖基转移酶 UGT76F2（At3g55710）可催化 IPA 的糖基化，从而调节 IAA 水平。我们仔细分析了 UGT76F2 及其近缘同源物 UGT76F1（At3g55700）在 IAA 平衡中的生理作用。我们通过CRISPR/Cas9基因编辑技术生成了两个独立的ugt76f1 ugt76f2双空拟南芥突变体（ugt76f1f2）和两个独立的ugt76f2单空突变体，前者缺失了2.7 kb。令人惊讶的是，在我们的实验室条件下，这些空突变体表现出与野生型幼苗无异的表型。我们的研究结果表明，UGT76F1和UGT76F2在通过IPA糖基化调节IAA生物合成的过程中并未发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.