Influence of metal oxides on biocompatibility of additively manufactured NiTi.

IF 1.6 4区医学 Q4 BIOPHYSICS

Biointerphases Pub Date : 2024-09-01 DOI:10.1116/6.0003665

Maria P Kwesiga, Roger J Guillory, Ali Gökhan Demir

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Abstract

In order to properly satisfy biomedical constraints for cardiovascular applications, additively manufactured NiTi scaffolds required further process and metallurgical engineering. Additively manufactured NiTi materials for cardiovascular use will have to undergo surface finishing in order to minimize negative surface interactions within the artery. In this study, we sought to understand biocompatibility from chemically etched additively manufactured NiTi scaffolds by laser powder bed fusion (LPBF). Although two distinct oxide films were created in the surface etching process (labeled CP-A and CP-B), no qualitative changes in microroughness were seen between the two conditions. CP-A possessed significantly less Ni at the surface (0.19 at. %) than the CP-B group (3.30 at. %), via x-ray photoelectron spectroscopy, alongside a concomitant shift in the O1 s peak presentation alluding to a greater formation of a Ni based oxide in the CP-B group. Our live dead staining revealed significant toxicity and reduced cellular attachment for the CP-B group, in addition to inducing more cell lysis (20.9 ± 5.1%), which was significantly increased when compared to CP-A (P < 0.01). Future practices of manufacturing NiTi scaffolds using LPBF should focus on producing surface films that are not only smooth, but free of cytotoxic Ni based oxides.

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金属氧化物对加成制造镍钛生物相容性的影响

为了适当满足心血管应用的生物医学限制，快速成型镍钛支架需要进一步的工艺和冶金工程。用于心血管的添加制造镍钛材料必须经过表面处理，以尽量减少动脉内的负表面相互作用。在这项研究中，我们试图通过激光粉末床熔融（LPBF）了解化学蚀刻加成法制造的镍钛支架的生物相容性。虽然在表面蚀刻过程中产生了两种不同的氧化膜（CP-A 和 CP-B），但两种条件下的微观粗糙度没有质的变化。通过 X 射线光电子能谱，CP-A 组的表面镍含量（0.19%）明显低于 CP-B 组（3.30%），同时 O1 s 峰也发生了移动，这表明 CP-B 组形成了更多的镍基氧化物。我们的活死细胞染色结果表明，CP-B 组具有明显的毒性，细胞附着力降低，此外还诱导了更多的细胞裂解（20.9 ± 5.1%），与 CP-A 组相比，细胞裂解率显著增加（P.

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来源期刊

Biointerphases 生物-材料科学：生物材料

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期刊介绍： Biointerphases emphasizes quantitative characterization of biomaterials and biological interfaces. As an interdisciplinary journal, a strong foundation of chemistry, physics, biology, engineering, theory, and/or modelling is incorporated into originated articles, reviews, and opinionated essays. In addition to regular submissions, the journal regularly features In Focus sections, targeted on specific topics and edited by experts in the field. Biointerphases is an international journal with excellence in scientific peer-review. Biointerphases is indexed in PubMed and the Science Citation Index (Clarivate Analytics). Accepted papers appear online immediately after proof processing and are uploaded to key citation sources daily. The journal is based on a mixed subscription and open-access model: Typically, authors can publish without any page charges but if the authors wish to publish open access, they can do so for a modest fee. Topics include: bio-surface modification nano-bio interface protein-surface interactions cell-surface interactions in vivo and in vitro systems biofilms / biofouling biosensors / biodiagnostics bio on a chip coatings interface spectroscopy biotribology / biorheology molecular recognition ambient diagnostic methods interface modelling adhesion phenomena.