UPLC-MS/MS method for fluconazole determination in plasma and its application in Mexican patients with candidaemia.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-05 DOI:10.1080/17576180.2024.2387452

Karla Paulina Valero Rivera, Martin Magaña Aquino, Julia Sagahón Azúa, María Del Carmen Romero Méndez, Susanna Edith Medellín Garibay, Rosa Del Carmen Milán Segovia, Fidel Martínez Gutiérrez, Silvia Romano Moreno

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Abstract

Aim: An accurate and fast ultra-high performance liquid chromatography coupled with tandem mass spectrometry analytical method was developed and validated for quantifying fluconazole levels in human plasma according to the US FDA guidelines.Materials & methods: A simple protein precipitation by acetonitrile was employed for the sample preparation. The chromatographic separation was carried out using isocratic elution of water (0.1% formic acid) and acetonitrile on an Acquity ultra-high performance liquid chromatography HSS T3 column. Samples from ten adult patients diagnosed with candidemia who received fluconazole treatment were analyzed.Results & conclusion: The method demonstrated excellent linearity and stability within the 1-50 μg/ml range (r² >0.999). The intraday and interday precision were determined with coefficient of variation values ranging from 1.4 to 4.38% and 2.8 to 6.6%, respectively. This rapid and selective method has successfully analyzed 27 plasma samples. The straightforward sample preparation in a single step and the reduced analysis time make this method suitable for adult patients with candidemia, leading to improved clinical outcomes.

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测定血浆中氟康唑含量的 UPLC-MS/MS 方法及其在墨西哥念珠菌血症患者中的应用

目的：根据美国 FDA 指南，建立并验证了一种准确、快速的超高效液相色谱-串联质谱分析方法，用于定量检测人体血浆中氟康唑的含量：样品制备采用简单的乙腈沉淀蛋白法。色谱分离采用 Acquity 超高效液相色谱 HSS T3 柱，用水（0.1% 甲酸）和乙腈进行等度洗脱。对 10 名接受氟康唑治疗的成年念珠菌病患者的样本进行了分析：该方法在 1-50 μg/ml 范围内具有良好的线性和稳定性（r2 >0.999）。日内精密度和日间精密度的变异系数分别为 1.4% 至 4.38% 和 2.8% 至 6.6%。该方法快速、选择性强，已成功分析了 27 份血浆样品。该方法只需一步即可完成样品制备，而且分析时间缩短，因此适用于成年念珠菌血症患者，从而改善了临床疗效。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.