Two types of amino acid substitutions in the succinate dehydrogenase complex subunit confer resistance to benzovindiflupyr in Colletotrichum sublineola.

IF 3.8 1区农林科学 Q1 AGRONOMY

Pest Management Science Pub Date : 2024-09-05 DOI:10.1002/ps.8406

Liyuan Deng, Weijin Sun, Yang Yu, Yuheng Yang, Anfei Fang, Binnian Tian, Jing Wang, Chaowei Bi

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引用次数: 0

Abstract

Background: Colletotrichum sublineola is the pathogenic fungus that causes sorghum anthracnose, which seriously threatens sorghum yield. Benzovindiflupyr is a succinate dehydrogenase inhibitor with good control effects on various crop diseases. However, the control of sorghum anthracnose by benzovindiflupyr and the risk of resistance to benzovindiflupyr in this pathogen are not well studied. Therefore, this study aimed to evaluate the benzovindiflupyr resistance and underlying mechanisms in C. sublineola.

Results: Analysis of the sensitivity of 126 C. sublineola strains to benzovindiflupyr revealed that the average EC₅₀ of the fungicide was 0.0503 ± 0.0189 μg mL^-1, with a unimodal normal distribution curve. The survival fitness of 10 benzovindiflupyr-resistant strains decreased to varying degrees compared with that of the wild-type parental strains. Additionally, a significant positive cross-resistance was observed between benzovindiflupyr and carboxin. Sequencing analyses identified two mutation sites, CsSdhB^H249Y and CsSdhC^G81V, in the resistant strains. Further molecular docking and site-directed mutagenesis experiments confirmed that the CsSdhB^H249Y and CsSdhC^G81V substitutions conferred resistance to benzovindiflupyr in C. sublineola.

Conclusion: Colletotrichum sublineola is sensitive to benzovindiflupyr and shows a moderate resistance risk to benzovindiflupyr. Two specific point substitutions, CsSdhB^H249Y and CsSdhC^G81V, are responsible for the resistance of C. sublineola to benzovindiflupyr. These findings offer a theoretical foundation for strategic application of the fungicide in controlling sorghum anthracnose, and for potentially delaying the emergence and progression of resistance. © 2024 Society of Chemical Industry.

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琥珀酸脱氢酶复合物亚基中的两种氨基酸替换赋予亚线轮孢霉对苯并茚虫威的抗性。

背景：高粱炭疽病的病原真菌是亚线形高粱轮纹霉菌（Colletotrichum sublineola），它严重威胁着高粱的产量。苯并茚虫威是一种琥珀酸脱氢酶抑制剂，对多种作物病害具有良好的防治效果。然而，苯并茚虫威对高粱炭疽病的防治效果以及该病原菌对苯并茚虫威产生抗性的风险尚未得到充分研究。因此，本研究旨在评估子线虫对苯并茚虫威的抗性及其内在机制：结果：分析了 126 株 C. sublineola 对苯并嘧啶氟吡啶的敏感性，发现该杀菌剂的平均 EC50 为 0.0503 ± 0.0189 μg mL-1，呈单峰正态分布曲线。与野生型亲本菌株相比，10株苯并嘧啶氟吡啶抗性菌株的存活适宜性均有不同程度的下降。此外，苯并茚虫威和羧草醚之间还出现了明显的正交抗性。测序分析在抗性菌株中发现了两个突变位点，即 CsSdhBH249Y 和 CsSdhCG81V。进一步的分子对接和定点诱变实验证实，CsSdhBH249Y 和 CsSdhCG81V 的置换位点使亚线虫对苯并茚虫威产生抗性：结论：Colletotrichum sublineola 对苯并嘧啶氟吡啶敏感，对苯并嘧啶氟吡啶有中度抗性风险。CsSdhBH249Y和CsSdhCG81V这两个特定的点置换是导致亚线虫对苯并茚虫威产生抗性的原因。这些发现为战略性地使用杀菌剂防治高粱炭疽病，以及延缓抗药性的产生和发展提供了理论依据。© 2024 化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pest Management Science 农林科学-昆虫学

CiteScore

7.90

自引率

9.80%

发文量

553

审稿时长

4.8 months

期刊介绍： Pest Management Science is the international journal of research and development in crop protection and pest control. Since its launch in 1970, the journal has become the premier forum for papers on the discovery, application, and impact on the environment of products and strategies designed for pest management. Published for SCI by John Wiley & Sons Ltd.

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