Biocontrol of citrus melanose Diaporthe citri by Bacillus subtilis M23

IF 3.7 2区 农林科学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Zehua Zhou , Sheng Lu , Tiantian Liu , Jie Liu , Jiefu Deng , Xiaopeng Lu , Liangying Dai , Tuyong Yi
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Abstract

Citrus melanose, caused by Diaporthe citri, is one of the most prevalent and important fungal diseases of citrus crops globally. However, the overuse of chemical fungicides for disease control has an adverse impact on citrus production. In contrast, biological control agents (BCAs) are environmentally friendly and have become essential tools for plant disease control. In the present study, Bacillus subtilis M23, a strain isolated from healthy citrus leaves, significantly suppressed the mycelial growth of D. citri, the causal agent of citrus melanose, in vitro. The fermentation broth of B. subtilis M23 also exhibited good antifungal activity against D. citri on citrus plants in greenhouse and field experiments. Lipopeptides (LPs) produced by M23 exhibited excellent antifungal activity against D. citri, with a 50 % effective concentration (EC50) of approximately 1.00 and 0.28 μg/mL for inhibition of mycelial growth and conidial germination, respectively. LP biosynthesis genes were identified in the M23 genome using a PCR assay. Notably, LP extracts significantly reduced the formation of pycnidia and ATP biosynthesis in D. citri. Comparative transcriptome analysis revealed significant differences in the expression of genes associated with fungal vacuole, oxidoreductase activity and endopeptidase activity in D. citri treated with B. subtilis M23. Both RNA-seq and RT-qPCR analysis provided similar results on the expression of selected genes. Collectively, our data provided convincing data supporting the biocontrol potential of B. subtilis M23 for the management of citrus melanose.

枯草芽孢杆菌 M23 对柑橘黑点病 Diaporthe citri 的生物防治
由柑橘黑腐病(Diaporthe citri)引起的柑橘黑腐病是全球柑橘作物中最普遍、最重要的真菌病害之一。然而,过度使用化学杀菌剂控制病害对柑橘生产造成了不利影响。相比之下,生物防治剂(BCA)对环境友好,已成为植物病害防治的重要工具。在本研究中,从柑橘健康叶片中分离出的枯草芽孢杆菌 M23 能在体外显著抑制柑橘黑色素病原菌 D. citri 的菌丝生长。在温室和田间试验中,枯草芽孢杆菌 M23 的发酵液对柑橘植物上的柠檬褐斑病菌也表现出良好的抗真菌活性。M23 产生的脂肽(LPs)对柑橘褐斑病菌有很好的抗真菌活性,抑制菌丝生长和分生孢子萌发的 50 % 有效浓度(EC50)分别约为 1.00 和 0.28 μg/mL。利用聚合酶链式反应(PCR)方法在 M23 基因组中确定了 LP 生物合成基因。值得注意的是,LP 提取物明显减少了柠檬蝇菌丝的形成和 ATP 的生物合成。比较转录组分析表明,用枯草芽孢杆菌 M23 处理的柠檬蝙蝠蛾中,与真菌液泡、氧化还原酶活性和内肽酶活性相关的基因表达存在显著差异。RNA-seq 和 RT-qPCR 分析对所选基因的表达提供了相似的结果。总之,我们的数据提供了令人信服的数据,支持枯草芽孢杆菌 M23 在治理柑橘黑色素方面的生物防治潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Biological Control
Biological Control 生物-昆虫学
CiteScore
7.40
自引率
7.10%
发文量
220
审稿时长
63 days
期刊介绍: Biological control is an environmentally sound and effective means of reducing or mitigating pests and pest effects through the use of natural enemies. The aim of Biological Control is to promote this science and technology through publication of original research articles and reviews of research and theory. The journal devotes a section to reports on biotechnologies dealing with the elucidation and use of genes or gene products for the enhancement of biological control agents. The journal encompasses biological control of viral, microbial, nematode, insect, mite, weed, and vertebrate pests in agriculture, aquatic, forest, natural resource, stored product, and urban environments. Biological control of arthropod pests of human and domestic animals is also included. Ecological, molecular, and biotechnological approaches to the understanding of biological control are welcome.
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