Structural characterization and immune-enhancing properties of an acidic heteroglycan from Protaetia brevitarsis seulensis larvae

IF 7.2 Q1 FOOD SCIENCE & TECHNOLOGY
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引用次数: 0

Abstract

Edible insects are a promising source of bioactive compounds with diverse health benefits. This study investigated the immunomodulatory properties of polysaccharides isolated from Protaetia brevitarsis seulensis larvae (PBSL). Polysaccharides were fractionated using a molecular weight cutoff membrane, and among these fractions, the higher molecular weight fraction (H-PBSL, 666.84 kDa) demonstrated significant immunomodulatory activity. Methylation and NMR structural analyses revealed H-PBSL to be an acidic heteroglycan with a complex structure, consisting of a backbone of repeating (1→4)-linked α-glucan units highly branched at O-6 positions with T-α-Glcp, T-α-GlcpA, and T-β-Galp. The immunomodulatory effects of H-PBSL were evaluated using RAW264.7 macrophage cells. H-PBSL treatment enhanced phagocytic activity and increased the expression of immune modulators and macrophage activation surface molecules. In addition, H-PBSL activated the NF-κB signaling pathway, evidenced by increased phosphorylation of IκB and NF-κB, and translocation of NF-κB from cytosol to nucleus. Moreover, inhibition of Toll-like receptor 4 (TLR4) reduced H-PBSL-induced nitric oxide synthesis, indicating TLR4′s pivotal role in H-PBSL-mediated macrophage activation. These findings highlight the potential of H-PBSL as a novel immunomodulatory agent derived from an edible insect, with possible applications in functional foods and nutraceuticals.

一种产自海鲂幼虫的酸性异聚糖的结构特征和免疫增强特性
食用昆虫是具有多种健康益处的生物活性化合物的重要来源。本研究调查了从Protaetia brevitarsis seulensis幼虫(PBSL)中分离出的多糖的免疫调节特性。使用分子量截止膜对多糖进行了分馏,其中分子量较高的部分(H-PBSL,666.84 kDa)具有显著的免疫调节活性。甲基化和核磁共振结构分析表明,H-PBSL 是一种酸性异聚糖,结构复杂,由重复(1→4)连接的α-葡聚糖单位骨架组成,在 O-6 位高度支化,含有 T-α-Glcp、T-α-GlcpA 和 T-β-Galp。使用 RAW264.7 巨噬细胞评估了 H-PBSL 的免疫调节作用。H-PBSL 处理增强了吞噬活性,并增加了免疫调节剂和巨噬细胞活化表面分子的表达。此外,H-PBSL 还激活了 NF-κB 信号通路,表现为 IκB 和 NF-κB 磷酸化增加,NF-κB 从细胞膜转位到细胞核。此外,抑制 Toll 样受体 4(TLR4)可减少 H-PBSL 诱导的一氧化氮合成,这表明 TLR4 在 H-PBSL 介导的巨噬细胞活化中起着关键作用。这些发现凸显了 H-PBSL 作为一种从可食用昆虫中提取的新型免疫调节剂的潜力,并有可能应用于功能食品和营养保健品中。
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来源期刊
Future Foods
Future Foods Agricultural and Biological Sciences-Food Science
CiteScore
8.60
自引率
0.00%
发文量
97
审稿时长
15 weeks
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