The uplift of the Qinghai-Tibetan Plateau (QTP) is one of the most significant geological events in the Cenozoic era. While most studies have focused on the latitudinal differences in the uplift process of the QTP, there has been scant attention to its longitudinal differentiation. The Miocene epoch is pivotal for understanding both the uplift of the QTP and associated climatic changes. Wildfire events not only record changes in vegetation composition but also reflect climatic fluctuations and their driving forces. However, investigations into the interactions among these factors remain limited. This study aims to explore the coupling between the uplift of the QTP, climatic changes and wildfire frequency (or intensity) from northeastern QTP by analyzing microcharcoal concentrations and length-to-width ratios from the Miocene Youshashan Formation in Wulan County, Qinghai Province. The results indicate that the development of wildfires could be divided into three stages. Compared with the intervals 18–15 Ma and 11–8.7 Ma, the middle stage (15–11 Ma) experienced the highest wildfire frequency. This finding underscores the synchronous and close relationship between wildfire occurrences, the uplift of the QTP, and consequent climatic fluctuations. The ratio of length-to-width of microcharcoal indicates that Miocene wildfires in the Wulan Basin primarily occurred at the transitional zones between forests and grasslands. Moreover, the highest peak of wildfire events at six sites gradually shifted from the northeastern to the northwestern QTP from 18–8.7 Ma. This fact demonstrates spatiotemporal disparities in wildfire events from northern QTP, likely stemming from asynchronous uplifts there.