The effect of eight weeks of endurance training and MitoQ supplementation on antioxidant capacity and the expression of sestrin-2 and AMPK in cardiac tissue of aged rats

IF 3.9

Experimental gerontology Pub Date : 2024-09-04 DOI:10.1016/j.exger.2024.112572

{"title":"The effect of eight weeks of endurance training and MitoQ supplementation on antioxidant capacity and the expression of sestrin-2 and AMPK in cardiac tissue of aged rats","authors":"","doi":"10.1016/j.exger.2024.112572","DOIUrl":null,"url":null,"abstract":"<div><h3>Purpose</h3><p>The present study aimed to investigate the effects of endurance training (ET) in combination with MitoQ supplementation on antioxidant indices and the expression of sesterin-2 (SESN2) as an anti-aging factor and AMPK as an energy sensor in aged male Wistar rats.</p></div><div><h3>Methods</h3><p>Twenty-eight aged Wistar rats (410 ± 15 g, 22 ± 1.5 months old) were randomly divided into four groups (<em>n</em> = 7): Control, ET (eight weeks endurance training on the treadmill), MitoQ (250 μ/L in drinking water), and ET + MitoQ. We measured the protein and gene expression of SESN2 and AMPK in the heart tissue by western blotting and real-time PCR, respectively. In addition, antioxidant indices, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activity, and oxidant malondialdehyde (MDA) concentration in the cardiac tissue and serum were measured.</p></div><div><h3>Results</h3><p>SESN2 and AMPK protein expression significantly increased in the MitoQ group compared to the control group (<em>P =</em> 0.002, <em>P =</em> 0.0003). MDA content in tissue and serum remained unchanged in all groups (<em>P ></em> 0.05). MitoQ supplementation significantly increased SOD and GPx enzyme activity in serum and cardiac tissue (<em>P =</em> 0.001).</p></div><div><h3>Conclusion</h3><p>Overall, ET and MitoQ alone and in combination have anti-aging effects and improve the expression of AMPK and SESN2. Additionally, ET and MitoQ lead to improved antioxidant capacity in aged rats by ameliorating the activity of antioxidant enzymes.</p></div>","PeriodicalId":94003,"journal":{"name":"Experimental gerontology","volume":null,"pages":null},"PeriodicalIF":3.9000,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0531556524002183/pdfft?md5=3ab9c6e94b5be6275147b38f25d1b387&pid=1-s2.0-S0531556524002183-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Experimental gerontology","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0531556524002183","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

Abstract

Purpose

The present study aimed to investigate the effects of endurance training (ET) in combination with MitoQ supplementation on antioxidant indices and the expression of sesterin-2 (SESN2) as an anti-aging factor and AMPK as an energy sensor in aged male Wistar rats.

Methods

Twenty-eight aged Wistar rats (410 ± 15 g, 22 ± 1.5 months old) were randomly divided into four groups (n = 7): Control, ET (eight weeks endurance training on the treadmill), MitoQ (250 μ/L in drinking water), and ET + MitoQ. We measured the protein and gene expression of SESN2 and AMPK in the heart tissue by western blotting and real-time PCR, respectively. In addition, antioxidant indices, superoxide dismutase (SOD), and glutathione peroxidase (GPx) activity, and oxidant malondialdehyde (MDA) concentration in the cardiac tissue and serum were measured.

Results

SESN2 and AMPK protein expression significantly increased in the MitoQ group compared to the control group (P = 0.002, P = 0.0003). MDA content in tissue and serum remained unchanged in all groups (P > 0.05). MitoQ supplementation significantly increased SOD and GPx enzyme activity in serum and cardiac tissue (P = 0.001).

Conclusion

Overall, ET and MitoQ alone and in combination have anti-aging effects and improve the expression of AMPK and SESN2. Additionally, ET and MitoQ lead to improved antioxidant capacity in aged rats by ameliorating the activity of antioxidant enzymes.

查看原文本刊更多论文

八周耐力训练和补充 MitoQ 对老龄大鼠心脏组织抗氧化能力以及胰蛋白酶 2 和 AMPK 表达的影响

目的本研究旨在探讨耐力训练（ET）与补充线粒体质量（MitoQ）相结合对老年雄性 Wistar 大鼠抗氧化指数、抗衰老因子 Sesterin-2 (SESN2) 和能量传感器 AMPK 表达的影响。方法将 28 只老年 Wistar 大鼠（410 ± 15 g，22 ± 1.5 月龄）随机分为四组（n = 7）：对照组、ET 组（在跑步机上进行八周耐力训练）、MitoQ 组（在饮用水中添加 250 μ/L）和 ET + MitoQ 组。我们分别用 Western 印迹法和实时 PCR 法检测了心脏组织中 SESN2 和 AMPK 的蛋白和基因表达。此外，还测定了心脏组织和血清中的抗氧化指数、超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）活性以及氧化剂丙二醛（MDA）浓度。各组组织和血清中的 MDA 含量均保持不变（P > 0.05）。总之，单独或联合使用 ET 和 MitoQ 有抗衰老作用，并能改善 AMPK 和 SESN2 的表达。此外，ET 和 MitoQ 还能改善抗氧化酶的活性，从而提高老龄大鼠的抗氧化能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Experimental gerontology Ageing, Biochemistry, Geriatrics and Gerontology

CiteScore

6.70

自引率

0.00%

发文量

审稿时长

66 days