Versatile effects of galectin-1 protein-containing lipid bilayer coating for cardiovascular applications

IF 18 1区医学 Q1 ENGINEERING, BIOMEDICAL

Bioactive Materials Pub Date : 2024-09-03 DOI:10.1016/j.bioactmat.2024.08.026

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引用次数: 0

Abstract

Modulating inflammatory cells in an implantation site leads to severe complications and still unsolved challenges for blood-contacting medical devices. Inspired by the role of galectin-1 (Gal-1) in selective functions on multiple cells and immunomodulatory processes, we prepared a biologically target-specific surface coated with the lipid bilayer containing Gal-1 (Gal-1-SLB) and investigate the proof of the biological effects. First, lipoamido-dPEG-acid was deposited on a gold-coated substrate to form a self-assembled monolayer and then conjugated dioleoylphosphatidylethanolamine (DOPE) onto that to produce a lower leaflet of the supported lipid bilayer (SLB) before fusing membrane-derived vesicles extracted from B16-F10 cells. The Gal-1-SLB showed the expected anti-fouling activity by revealing the resistance to protein adsorption and bacterial adhesion. In vitro studies showed that the Gal-1-SLB can promote endothelial function and inhibit smooth muscle cell proliferation. Moreover, Gal-1- SLB presents potential function for endothelial cell migration and angiogenic activities. In vitro macrophage culture studies showed that the Gal-1-SLB attenuated the LPS-induced inflammation and the production of macrophage-secreted inflammatory cytokines. Finally, the implanted Gal-1-SLB reduced the infiltration of immune cells at the tissue-implant interface and increased markers for M2 polarization and blood vessel formation in vivo. This straightforward surface coating with Gal-1 can be a useful strategy for modulating the vascular and immune cells around a blood-contacting device.

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含 galectin-1 蛋白的脂质双分子层涂层在心血管领域的多种应用效果

调节植入部位的炎症细胞会导致严重的并发症，这对于与血液接触的医疗器械来说仍是一个尚未解决的难题。受 galectin-1 (Gal-1) 对多种细胞的选择性作用和免疫调节过程的启发，我们制备了一种涂有含 Gal-1 的脂质双分子层（Gal-1-SLB）的生物靶标特异性表面，并研究了其生物效应的证明。首先，在金涂层基底上沉积脂酰胺基-dPEG-酸，形成自组装单层，然后在其上共轭二油酰磷脂酰乙醇胺（DOPE），形成支撑脂质双分子层（SLB）的下层小叶，最后融合从B16-F10细胞中提取的膜源小泡。Gal-1-SLB具有抗蛋白质吸附和细菌粘附的能力，因而具有预期的抗污活性。体外研究表明，Gal-1-SLB 可以促进内皮功能，抑制平滑肌细胞增殖。此外，Gal-1- SLB 还具有促进内皮细胞迁移和血管生成的潜在功能。体外巨噬细胞培养研究表明，Gal-1-SLB 可减轻 LPS 诱导的炎症反应和巨噬细胞分泌的炎症细胞因子。最后，植入的 Gal-1-SLB 减少了免疫细胞在组织-植入物界面的浸润，增加了体内 M2 极化和血管形成的标记物。这种直接在表面涂覆 Gal-1 的方法是调节血液接触装置周围血管和免疫细胞的有效策略。

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来源期刊

Bioactive Materials Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

28.00

自引率

6.30%

发文量

436

审稿时长

20 days

期刊介绍： Bioactive Materials is a peer-reviewed research publication that focuses on advancements in bioactive materials. The journal accepts research papers, reviews, and rapid communications in the field of next-generation biomaterials that interact with cells, tissues, and organs in various living organisms. The primary goal of Bioactive Materials is to promote the science and engineering of biomaterials that exhibit adaptiveness to the biological environment. These materials are specifically designed to stimulate or direct appropriate cell and tissue responses or regulate interactions with microorganisms. The journal covers a wide range of bioactive materials, including those that are engineered or designed in terms of their physical form (e.g. particulate, fiber), topology (e.g. porosity, surface roughness), or dimensions (ranging from macro to nano-scales). Contributions are sought from the following categories of bioactive materials: Bioactive metals and alloys Bioactive inorganics: ceramics, glasses, and carbon-based materials Bioactive polymers and gels Bioactive materials derived from natural sources Bioactive composites These materials find applications in human and veterinary medicine, such as implants, tissue engineering scaffolds, cell/drug/gene carriers, as well as imaging and sensing devices.