Evaluation of a whole blood point-of-care coagulation analyzer in dogs

IF 1.1 3区 农林科学 Q3 VETERINARY SCIENCES
Elizabeth M. Conroy VMD, Bridget M. Lyons VMD, DACVECC, Amie Koenig DVM, DACVIM, DACVECC
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引用次数: 0

Abstract

Objective

To compare the accuracy of a point-of-care coagulation analyzer (POCCA) with a reference laboratory coagulation analyzer (LabCA) and to evaluate for confounding factors that could alter the performance of the POCCA.

Design

Prospective, observational study.

Setting

Two university veterinary teaching hospitals.

Animals

Forty-three client-owned dogs undergoing coagulation testing between April 2020 and June 2021.

Methods

Samples were obtained from dogs undergoing coagulation testing as part of a diagnostic workup. Prothrombin time (PT) and activated partial thromboplastin time (aPTT) were measured on the POCCA and on the LabCA. PCV, platelet count, total plasma protein, hyperbilirubinemia, hemolysis, lipemia, and autoagglutination were recorded.

Results

Moderate correlation was seen for PT and strong correlation was seen for aPTT between the POCCA and the LabCA (PT: 0.59, P < 0.0001; aPTT: 0.71, P < 0.0001). The POCCA results were consistent with normal or hypocoagulable samples for 30 of 38 PT and 33 of 37 aPTT results, as identified by the LabCA. Samples with PCV of 30%–55% were moderately correlated (PT: 0.63, P = 0.0004; aPTT: 0.63, P = 0.0003), but those outside that range were more likely to register an error message on the POCCA or provide disparate results. When hemolysis was present, there was a weak correlation between the POCCA and the LabCA for PT (rho: 0.38 [95% confidence interval: 0.19–0.76], P = 0.18) and a strong correlation for aPTT (rho: 0.86 [95% confidence interval: 0.62–0.95], P < 0.0001). Samples with hyperbilirubinemia were strongly correlated for PT (0.97, P = 0.002) but not for aPTT. Lipemia and autoagglutination were not observed.

Conclusion

There was an acceptable correlation in patients with PCV within the manufacturer's recommended reference range; however, measurements on samples with PCV outside the reference range were inconsistent with the LabCA. Caution should be used when using the POCCA in patients with coagulopathy and anemia or other potential confounders.

评估狗全血护理点凝血分析仪。
目的比较床旁凝血分析仪(POCCA)与参考实验室凝血分析仪(LabCA)的准确性,并评估可能改变床旁凝血分析仪性能的混杂因素:设计:前瞻性观察研究:地点:两所大学兽医教学医院:在 2020 年 4 月至 2021 年 6 月期间接受凝血检测的 43 只客户自养犬:作为诊断工作的一部分,从接受凝血检测的狗身上采集样本。在 POCCA 和 LabCA 上测量凝血酶原时间(PT)和活化部分凝血活酶时间(aPTT)。记录 PCV、血小板计数、血浆总蛋白、高胆红素血症、溶血、脂血和自身凝集素:结果:POCCA 和 LabCA 之间的 PT 呈中度相关,aPTT 呈高度相关(PT:0.59,P 结论:POCCA 和 LabCA 之间的 PT 和 aPTT 呈高度相关:对于 PCV 在制造商推荐参考范围内的患者,其相关性是可以接受的;但对于 PCV 超出参考范围的样本,其测量结果与 LabCA 不一致。在有凝血病、贫血或其他潜在混杂因素的患者中使用 POCCA 时应谨慎。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.50
自引率
15.40%
发文量
121
审稿时长
18-36 weeks
期刊介绍: The Journal of Veterinary Emergency and Critical Care’s primary aim is to advance the international clinical standard of care for emergency/critical care patients of all species. The journal’s content is relevant to specialist and non-specialist veterinarians practicing emergency/critical care medicine. The journal achieves it aims by publishing descriptions of unique presentation or management; retrospective and prospective evaluations of prognosis, novel diagnosis, or therapy; translational basic science studies with clinical relevance; in depth reviews of pertinent topics; topical news and letters; and regular themed issues. The journal is the official publication of the Veterinary Emergency and Critical Care Society, the American College of Veterinary Emergency and Critical Care, the European Veterinary Emergency and Critical Care Society, and the European College of Veterinary Emergency and Critical Care. It is a bimonthly publication with international impact and adheres to currently accepted ethical standards.
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