PRL1 interacts with and stabilizes RPA2A to regulate carbon deprivation-induced senescence in Arabidopsis

IF 8.3 1区生物学 Q1 PLANT SCIENCES

New Phytologist Pub Date : 2024-09-04 DOI:10.1111/nph.20082

Jingjing Meng, Wenhui Zhou, Xinhao Mao, Pei Lei, Xue An, Hui Xue, Yafei Qi, Fei Yu, Xiayan Liu

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引用次数: 0

Abstract

Leaf senescence is a developmental program regulated by both endogenous and environmental cues. Abiotic stresses such as nutrient deprivation can induce premature leaf senescence, which profoundly impacts plant growth and crop yield. However, the molecular mechanisms underlying stress-induced senescence are not fully understood.
In this work, employing a carbon deprivation (C-deprivation)-induced senescence assay in Arabidopsis seedlings, we identified PLEIOTROPIC REGULATORY LOCUS 1 (PRL1), a component of the NineTeen Complex, as a negative regulator of C-deprivation-induced senescence.
Furthermore, we demonstrated that PRL1 directly interacts with the RPA2A subunit of the single-stranded DNA-binding Replication Protein A (RPA) complex. Consistently, the loss of RPA2A leads to premature senescence, while increased expression of RPA2A inhibits senescence. Moreover, overexpression of RPA2A reverses the accelerated senescence in prl1 mutants, and the interaction with PRL1 stabilizes RPA2A under C-deprivation.
In summary, our findings reveal the involvement of the PRL1-RPA2A functional module in C-deprivation-induced plant senescence.

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PRL1 与 RPA2A 相互作用并稳定 RPA2A，以调节拟南芥中碳剥夺诱导的衰老。

叶片衰老是一种受内源和环境因素调控的发育程序。养分匮乏等非生物胁迫可诱导叶片过早衰老，从而对植物生长和作物产量产生深远影响。然而，人们对胁迫诱导衰老的分子机制还不完全了解。在这项工作中，我们利用拟南芥幼苗碳剥夺（C-drivation）诱导衰老试验，确定了 NineTeen 复合体的一个组分 PLEIOTROPIC REGULATORY LOCUS 1（PRL1）是 C-drivation诱导衰老的负调控因子。此外，我们还证明了 PRL1 直接与单链 DNA 结合复制蛋白 A（RPA）复合物的 RPA2A 亚基相互作用。一致的是，RPA2A的缺失会导致过早衰老，而增加RPA2A的表达则会抑制衰老。此外，过量表达 RPA2A 可逆转 prl1 突变体中的加速衰老，与 PRL1 的相互作用可稳定 C 缺失下的 RPA2A。总之，我们的发现揭示了 PRL1-RPA2A 功能模块参与了 C 缺失诱导的植物衰老。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.