Antioxidant effects and compatibility of zinc oxide nanoparticles during in vitro maturation of bovine oocytes and subsequent embryo development

IF 2.4 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY
Carolina C.R. Quintão , Naiara Z. Saraiva , Clara S. Oliveira , Elaine C. Paris , Luiz S.A. Camargo , Humberto M. Brandão , Michele Munk
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引用次数: 0

Abstract

Zinc oxide nanoparticles (ZnO-NPs) have garnered significant attention in biological applications due to their known antioxidant properties. However, their potential impact on assisted reproduction techniques remains largely unexplored, particularly in the context of oocyte quality maintenance within in vitro culture systems, where free radicals can exert detrimental effects. This study investigated the effects of incorporating ZnO-NPs to in vitro maturation (IVM) media on the developmental, cryosurvival, and metabolic profiles of bovine embryos. Three concentrations of ZnO-NPs (0, 1.0, and 1.5 μg/mL) were evaluated. We observed, for the first time, that the inclusion of ZnO-NPs at a concentration of 1.0 μg/mL led to a significant increase in the number of embryonic cells (p < 0.05) accompanied by a reduction in reactive oxygen species production (p < 0.05). Notably, ZnO-NPs did not alter embryonic development, cryosurvival rates, or mitochondrial viability. These findings suggested that ZnO-NPs has antioxidant properties and are compatible with bovine oocytes. Consequently, they may serve as promising supplements to the IVM media, potentially enhancing the efficiency of assisted reproduction techniques.

氧化锌纳米颗粒在牛卵母细胞体外成熟和随后胚胎发育过程中的抗氧化作用和兼容性。
氧化锌纳米粒子(ZnO-NPs)因其众所周知的抗氧化特性而在生物应用中备受关注。然而,它们对辅助生殖技术的潜在影响在很大程度上仍未得到探索,尤其是在体外培养系统中卵母细胞质量的维持方面,因为自由基会产生有害影响。本研究调查了在体外成熟(IVM)培养基中加入 ZnO-NPs 对牛胚胎的发育、低温存活和新陈代谢的影响。我们评估了三种浓度的 ZnO-NPs(0、1.0 和 1.5 μg/mL)。我们首次观察到,加入浓度为 1.0 μg/mL 的 ZnO-NPs 能显著增加胚胎细胞的数量(p<0.05)。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
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